精品文档---下载后可任意编辑中国汉族 TMEM39A 基因单核苷酸多态性与系统性红斑狼疮的关联讨论的开题报告Title: Investigating the association between the single nucleotide polymorphism of TMEM39A gene in Chinese Han population and systemic lupus erythematosusIntroduction:Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that can affect multiple organs and systems in the body, including skin, joints, kidneys, and the nervous system. The complex nature of SLE involves genetic, environmental, and hormonal factors. Recent studies suggest that genetic factors play a significant role in the pathogenesis of SLE, and several genes have been identified as potential susceptibility loci for SLE.One such gene is TMEM39A, which encodes a transmembrane protein that is involved in the regulation of calcium channels and intracellular calcium concentration. A previous study has reported that a single nucleotide polymorphism (SNP) in TMEM39A gene (rs1132200) was significantly associated with SLE in a Korean population. However, the association between this SNP and SLE in the Chinese Han population has not been investigated.Objectives:The objectives of this study are as follows:1. To analyze the distribution of TMEM39A gene SNP (rs1132200) in the Chinese Han population.2. To investigate the association between TMEM39A gene SNP (rs1132200) and SLE in the Chinese Han population.Methodology:This study will include a case-control design. A total of 200 SLE patients and 200 healthy controls will be recruited from hospitals and clinics in China. The inclusion criteria for SLE patients will be based on the criteria established by the American College of Rheumatology. Healthy controls will be selected based on a self-reported lack of autoimmune diseases. Genomic DNA will be extracted from peripheral blood 精品文档---下载后可任意编辑leukocytes of all participants. The TMEM39A gene SNP (rs1132200) will be genotyped using the TaqMan SNP genotyping assay.Data analysis:Data will be analyzed using frequency analysis, chi-square tests, and logistic regression analysis. The results will be considered significant if the p-value is less than 0.05.Expected outcomes:The findings of this study will provide information on the association between the TMEM39A gene SNP (rs1132200) and SLE in the Chinese Han population. The results of this study could potentially contribute to a better understanding of the genetic factors involved in the pathogenesis of SLE in this population.
