探讨白念珠菌基因多态性与耐药性关系【摘要】 【目的】 探讨耐氟康唑白念珠菌基因多态性与其耐药性是否相关。【方法】 收集 48 株敏感白念珠菌和10 株耐氟康唑白念珠菌，其中包括 2 株体外诱导的耐氟康唑白念珠菌。选择 1 个随机引物，采纳任意引物 PCR 方法基因分型，将电泳图谱扫描入计算机后采纳软件转化为数值图表,利用 SPSS 进行聚类分析。 【结果】 引物 1251 的 PCR 指纹图带型稳定，多态性丰富，可以作为分型引物。聚类分析结果提示 8 株临床耐药菌的 PCR 指纹图相似系数为%，而其中 7 株的耐药菌相似系数高达%，远高于 58 株菌的平均相似系数(%)。2 株体外诱导的耐药菌诱导前后基因型未发生变化。【结论】 基因分型的结果未提示耐氟康唑白念珠菌存在一定的特别电泳条带，但提示了特定的 PCR 指纹图可能与耐药性有一定相关性。【关键词】 白念珠菌； 基因型； 氟康唑； 耐药性，任意引物 PCR Abstract： 【Objectives】 To investigate the possible relationship of C. albicans gene polymorphisms and its fluconazole-resistance. 【Method】 Forty-eight fluconazole-susceptible stains and 10 fluconazole-resistant stains of C. albicans were analyzed, with two resistant stains induced in vitro. All of them were genotyped by arbitrary primed polymerase chain reaction (AP-PCR) fingerprinting employing one interrepeat primes (1251 primers). The image was captured using computer-assisted system with Labwork software to analyze the gel patterns of all isolates. The data were processed by SPSS statistical software. 【Results】 Primer 1251 was suitable for fingerprinting analysis, and was found to generate the reproducible fingerprinting profiles, yielding well-resolved banding patterns. The AP-PCR profiles of 8 resistant stains isolated clinically were highly similar according to similarity coefficient (%). Furthermore, the similarity coefficient of 7 resistant isolates (%) was significantly higher than that of total 58 stains (%). For two induced isolates, their...
