目录第一章前言......................................................................................................................31.1胚胎干细胞神经分化概述.................................................................................31.2类糖胺聚糖对胚胎干细胞神经分化的影响................................................31.3金纳米粒子（AuNPs）的性能........................................................................41.4课题提出.................................................................................................................5第二章实验部分............................................................................................................62.1试剂与仪器...........................................................................................................62.2金纳米粒子聚合物的制备.................................................................................82.2.1MAG的制备.................................................................................................82.2.2MAG的RAFT聚合...................................................................................82.2.2.1PMAG的胺解...........................................................................................92.2.3SS的RAFT聚合.........................................................................................92.2.3.1PSS的胺解..........................................................................................102.2.4AuNPs的制备................................................................................102.2.5类糖胺聚糖改性AuNPs的制备...................................................112.3聚合物及其改性AuNPs的表征....................................................................112.3.1核磁共振氢谱（1H-NMR）测试..................................................112.3.2气相凝胶渗透色谱（GPC）测试................................................122.3.3动态光散射（DLS）测试.............................................................122.3.4透射电镜（TEM）........................................................................122.3.5紫外可见光测试（UV-visible）...................................................122.4细胞实验...............................................................................................122.4.1细胞毒性测试................................................................................122.4.1.1L929细胞复苏.............................................................................132.4.1.2L929细胞传代与计数.................................................................132.4.1.3L929细胞种植.............................................................................142.4.1.4L929细胞毒性测试.....................................................................142.4.2胚胎干细胞（MECs）培养..........................................................142.4.2.1小鼠胚胎成纤维（MEF）细胞的复苏、传代与冻存.............152.4.2.2MEF饲养层细胞的准备.............................................................152.4.2.3MES细胞的复苏........................................................................162.4.2.4小鼠胚胎干细胞的分化培养.....................................................162.4.2.5免疫荧光染色.............................................................................162.4.2.6聚合链式反应（PCR）...........................................
