目录中文摘要...........................................................................................................1Abstract.............................................................................................................2内容提要...........................................................................................................4第1章选题构思.............................................................................................51.1课题设计背景.......................................................................................51.2设计目标及可行性分析.......................................................................6第2章实验部分.............................................................................................82.1试剂与仪器...........................................................................................82.2顺-1,2-环己烷二甲酸官能化的聚酰胺-胺树枝状大分子（PAMAM-CCA）的合成.........................................................................82.2PAMAM-CCA的酸敏感性测定..........................................................92.3PAMAM-CCA包载疏水抗肿瘤药物阿霉素（DOX）.....................92.4透明质酸复合纳米凝胶（PAMAM-CCA@HA-NGs）的制备......102.5复合纳米凝胶的降解性能.................................................................102.6载DOX的复合纳米凝胶的制备及其体外药物释放行为...............11第3章结果与讨论.......................................................................................123.1PAMAM-CCA的合成与表征............................................................123.2PAMAM-CCA的理化性能................................................................143.3PAMAM-CCA对DOX的包载..........................................................173.4PAMAM-CCA@HA-NGs复合纳米凝胶的制备..............................173.5复合纳米凝胶的降解性能.................................................................193.6复合纳米凝胶对DOX的包载与释放...............................................20第4章总结与展望.......................................................................................224.1本文总结.............................................................................................224.2研究展望.............................................................................................22参考文献.........................................................................................................24致谢.................................................................................................................27附录.................................................................................................................28中文摘要恶性肿瘤严重威胁人类健康，纳米药物递送系统的出现给肿瘤的精准治疗带来了更多的可能性。聚酰胺-胺（PAMAM）树枝状高分子具有水溶性好、尺寸小（纳米级）和单分散性等特点，常被用作纳米药物载体。透明质酸是一种具有很好生物相容性、酶降解性、肿瘤靶向性的天然多糖，也常被用于肿瘤的靶向治疗。本文构建了含有顺-1,2-环己烷二甲酸官能化的PAMAM（PAMAM-CCA）的智能型透明质酸复合纳米凝胶（PAMAM-CCA@HA-NGs），用于憎水性抗肿瘤药物阿霉素（DOX）的有效包载和快速释放。动态光散射仪测试的PAMAM-CCA的Zeta电位变化曲线显示，在pH4.0的环境中分散的PAMAM-CCA的电位逐步升高，证实了PAMAM-CCA能在弱酸刺激下响应性脱除CCA，具有明显酸敏感特性。PAMAM-CCA@HA-NGs复合纳米凝胶的粒径在150nm左右，略大于HA-NGs纳米凝胶的...
