12MolecularCardiologyofChina，February2008，Vo1．8No．1(SerialNo．38SD大鼠生长发育期心脏结构及功能的超声心动图评价盖迎利徐明白燕吕志珍张幼怡李昭屏【摘要】目的应用高频超声仪动态观测SD雄性大鼠生长发育阶段心脏结构和功能变化规律，为发育期实验大鼠动物模型制备及功能评价提供依据。方法利用小动物超声仪连续观测sD雄性大鼠出生后1—9周左心室结构及功能的动态变化。结果(1)M超检测结果显示左室壁厚度及左室内径随生长发育而增加，超声评估左心室重量与解剖称重结果高度相关(r=0．9326，P<0．0001)；(2)左室短轴缩短率(FS％)在发育期无显著变化；(3)脉冲多普勒测定舒张早期峰值血流速度(E峰)与舒张晚期峰值血流速度(A峰)之比(E／A)>1，在发育过程中无显著性变化；(4)组织多普勒检测提示：左室后壁心肌的舒张早期(Em)、舒张晚期(Am)及收缩期(Sm)运动速度出生后1—6周逐渐增加，6周时达平台期。结论高频小动物超声仪经胸超声心动图所检测的心脏结构能准确地反映大鼠心脏生长发育变化趋势；心脏整体收缩及舒张功能在出生后1周即达稳定状态；组织多普勒检测左室后壁心肌纵轴运动在出生后逐渐成熟完善，于6周达到稳定状态。【关键词】心脏；生长发育；超声心动图；大鼠HighFrequencyUltrasoundAssessmentofDevelopmentalChangesofVentricularStructureandFunctioninSDRatGAIYing—i，XUMing2，BAIYan2，￡UZhi—zhen，ZhangYou—yi2，LIZhao·ping“．JDepartmentofCardiology，ThirdHospitalofBering100083，China．2InstituteofVascularMedi—cine，PekingUniversityThirdHospitalandKeyLaboratoryofMolecularCardiovascularSciences，MinistryofEducation，Beijing100083，ChinaCorrespondentauthor．L1Zhao-pingE—mail：lizhao-ping@sohu．corn【Abstract】0bjectiveToassessthedevelopmentalchangesofleftventricularstructureandfunctioninmaleSDratswi山ahighfrequencyultrasoundsystemandtoprovideevidenceforpreparationofratsmodelandfunctionalevaluation．MethodsUsingultrasonicinstrumentforsmallanimalsconsecutivelytoobserveleftventricularstructureandfunctionofmaleSDratsagedfrom1to9weeks．Results(1)M·modedetec·tionshowedthatleftventricularwallthicknessandleftventricularinsidediameterincreasedwith晤“g，U1·trasoundassessmentofleftventricularmassshowedahi．ghcorrelationwithanatomicalresults(r=0．9326，尸<0．0001)，(2)Therewasnosignificantchangeinleftventficularfractionalshortening(FS％)duringdevelopmentalperiod．(3)Ventficularfillingwasquantifiedasratioofearlypeakvelocity(Ewave)toatri-alpeakflowvelocity(Awave)，Inthecourseofdevelopment，E／Aratioishigherthan1，withnosignifi·cantchange．(4)TissueDopplerimagingwasappliedtomeasuremyocardialtissuevelocities：LVposteriorwallearlydiastolic(Em)，latediastolic(Am)andsystolic(Sm)myocardialtissuevelocitiesincreasedgraduallyfrom1to6weeksofageandreachedtoplatformat6-week．ConclusionsHighfrequencytran·sthoracicechocardiographyforsmallanimalsCanaccuratelyreflectgrowthanddevelopmentofratheartsbythedetectionofcardiacstructure．Cardiacsystolicanddiastolicfunctionsstablizeat1weekafterbirth．LeftventricularposteriorwalllongitudinalaxismovementdetectedbyTissueDopplerImagingmaturesgradually基金项目：国家自然科学基金资助项目(NO．30570716；30672466和国家重点基础研究发展规划项目计划(973计划，2006CB503806)作者单位：100083北京大学第三医院心内科(盖迎利、李昭屏)；北京大学第三医院血管医学研究所，分子血管学教育部重点实验室(徐明、白燕、吕志珍、张幼怡)通讯作者：李昭屏E-mail：lizhao·ping@sohu．eom．基础研究．维普资讯http://www.cqvip.com中国分子心脏病学杂志2008年2月第8卷第1期(总第38期afterbirthandstablizesinthe6thweek．【Keywords】Heart；Growthanddevelopment；Echocardiography；Rat...
