宜宾职业技术学院论文α-淀粉酶的生产工艺系部生物与化工工程系专业名称酿酒技术班级酿酒11103姓名王梦韵李艳梅严红俊学号201117315201117404201117346指导教师兰小艳二〇一二年十月二十九日α-淀粉酶的生产工艺摘要α-淀粉酶广泛分布于动物、植物和微生物中，能水解淀粉产生糊精、麦芽糖、低聚糖和葡萄糖等，是工业生产中应用最为广泛的酶制剂之一。目前，α-淀粉酶已广泛应用于变性淀粉及淀粉糖、焙烤工业、啤酒酿造、酒精工业、发酵以及纺织等许多行业。本次设计的淀粉酶发酵，分别以玉米粉为碳源，以豆饼为氮源，以BF-7658枯草芽孢杆菌为生产菌种,同时做出了生产工艺流程图，详细的介绍了α-淀粉酶的生产工艺。关键词：α-淀粉酶；生产工艺设计；深层发酵法AlphaamylaseproductiontechnologyAbstractAlphaamylasewidelydistributedinanimals,plantsandmicrobes,canbehydrolyzedstarchproducedextrin,maltose,oligosaccharideandglucoseandsoon,itistheindustrialproductioninthemostwidelyusedoneofenzymepreparation.Atpresent,thealphaamylasehasbeenwidelyusedinmodifiedstarchandstarchsugar,bakingindustry,brewing,alcoholindustry,hairleavenandtextileandmanyotherindustries.Thedesignofamylasefermentation,respectivelywithcornflourforcarbonsource,nitrogensourceforbeancake,withBF-7658bacillussubtilisstrainsfortheproduction,atthesametime,maketheproductionprocessflowdiagram,detailedintroducesthealphaamylaseproductiontechnology.Keywords:Alphaamylase,Productionprocessdesign;Deepfermentation目录前言................................................................................................................................11α-淀粉酶的生产方法..................................................................................................11.1生产方法的选择...................................................................................................11.2α-淀粉酶培养基的制作....................................................................31.2.1耐高温α-淀粉酶的生产方法...........................................................................41.2.2耐高温α－淀粉酶的生产原料.........................................................................41.2.3耐高温α－淀粉酶培养基制作.........................................................................51.2.4耐高温α－淀粉酶的另一种生产方法.............................................................51.2.5用菌种枯草芽孢杆菌来提取耐高温α－淀粉酶.............................................52α-淀粉酶的菌种选育......................................................................62.1菌株与培养方法.........................................................................................62.1.1培养基.......................................................................................................72.1.2仪器设备...................................................................................................72.2实验方法.......................................................................................................72.2.1细菌的分离与初步鉴定...................................................................72.2.2紫外线诱变育种..................................................................................72.3诱变方法以及变异菌株的筛选.......................................73α-淀粉酶分离纯化............................................................................................83.1α-淀粉酶的分离.........................................................................................83.2实验...
