课题名称血细胞染色方法的探讨摘要目的：本论文用不同的染色方法对血涂片染色，观察血细胞的形态，比较对血细胞的不同辨别结果。方法：通过Giemsa染色法、Wright染色法和Giemsa-Wright混合液染色3种染色方法对血涂片进行染色和血细胞的形态学观察。结果：在染色时间上Giemsa染液染色时间最长、Wright染液染色时间最短、Giemsa-Wright混合液染色时间介于两者之间，Giemsa染液的细胞核染色效果好，细胞质界限清晰，但细胞质中的颗粒染色效果较差；Wright染液染对细胞核和细胞质中的颗粒染色效果较好，细胞质的界限不淸；Giemsa-Wright混合染色具细胞核和细胞质中的颗粒染色效果较好，细胞界限清晰。结论：Giemsa染色法、Wright染色法和Giemsa-Wright混合染色三种染色方法均能有效的协助甄别血细胞，Giemsa-Wright混合染色方法相较于前两种方法染色效果更为明显，值得广泛应用。关键词：血细胞；血涂片；Giemsa染色法；Wright染色法；Giemsa-Wright混合染色法；细胞形态学ABSTRACTObjective:Inthispaper,differentstainingmethodswereusedtostainthebloodsmears,observethemorphologyofbloodcells,andcomparethedifferentresultsofbloodcells.Methods:ThebloodsmearswerestainedbyGiemsastaining,WrightstainingandGiemsa-Wrightstaining.Themorphologicalchangeswereobserved.Results:TheGiemsa-Wrightmixturehadtheshorteststainingtime,theGiemsa-Wrightmixturehadtheshortestdyeingtime,theGiemsa-Wrightmixturehadthesamestainingtime,andthecytoplasmicboundarieswereclear,butthecytoplasmwasclearThestainingeffectofWrightdyeonthenucleusandcytoplasmwasbetter,andthecytoplasmicboundarieswerenotclear.Giemsa-Wrightmixedstaininghadbetterstainingeffectonthenucleiandcytoplasm,andthecellboundarieswereclear.Conclusion:Giemsastainingmethod,WrightstainingmethodandGiemsa-Wrighthybridstainingmethodcaneffectivelyhelptoidentifybloodcells.Giemsa-Wrightmixedstainingmethodismoreobviousthanthefirsttwomethods,anditisworthytobewidelyused.KeyWords:bloodcells;bloodsmear;Giemsastaining;Wrightstaining;Giemsa-Wrightmixedstaining;cellmorphology目录摘要..................................................IABSTRACT.............................................II第一章绪论...........................................1第二章材料与方法.....................................22.1实验材料..............................................22.1.1主要仪器......................................................22.1.2试剂及药品....................................................22.1.3主要试剂的配制................................................22.2实验方法..............................................42.2.1血涂片的制作..................................................42.2.2Giemsa染色...................................................42.2.3Wright染色...................................................42.2.4Giemsa-Wright染色............................................42.2.5注意事项......................................................5第三章结果.........................................53.1Giemsa染色.....................................................53.2Wright染色.....................................................63.3Giemsa-Wright染色..............................................6第四章探究不同条件下三种染色方法的效果...............64.1不同染色条件下Giemsa的染色效果.................................64.2不同条件下Wright染液的染色效果................................74.3不同条件下Giemsa-Wright混合染液染色效果........................7第五章讨论.........................................74.1Giemsa氏染色法.............................................
