精品文档---下载后可任意编辑Claudin-4 mRNA 的降低可促进乳腺癌淋巴结转移的开题报告摘要：乳腺癌是世界范围内的一种常见癌症，淋巴结转移是其常见的临床表现之一。在癌细胞侵犯淋巴结时，细胞间紧密连接蛋白(claudins)可能发生改变。本文讨论团队在人乳腺癌细胞品系 MCF-7 中注射 siRNA 体外敲低 claudin-4 mRNA 并进行实验室体外和体内讨论，以探究淋巴结转移的相关性。结果显示，注射 siRNA 敲低 claudin-4 mRNA 释放了细胞间的联系，并增强颈部淋巴结转移（肿瘤细胞数比对比组增加66.7％）。该讨论揭示了 claudin-4 mRNA 的降低可不仅会促进乳腺癌细胞的迁移，更会影响淋巴结转移的发生率。关键词：claudin-4 mRNA，乳腺癌，淋巴结转移。Abstract:Breast cancer is a common cancer worldwide, and lymph node metastasis is one of its common clinical manifestations. When cancer cells invade lymph nodes, the tight junction protein (claudins) between cells may change. The research team injected siRNA into human breast cancer cell line MCF-7 to knock down claudin-4 mRNA and conducted laboratory in vitro and in vivo studies to explore the correlation of lymph node metastasis. The results showed that the injection of siRNA to knock down claudin-4 mRNA released the contact between cells and enhanced cervical lymph node metastasis (66.7% more tumor cells than the control group). This study revealed that the decrease of claudin-4 mRNA not only promotes the migration of breast cancer cells, but also affects the incidence of lymph node metastasis.Keywords: claudin-4 mRNA, breast cancer, lymph node metastasis.
