套式 PCR 结合基因测序鉴定象科动物 物种的研究学 院：专 业：姓 名：指导老师：学 号：职 称：中国·珠海二○二〇年五月北京理工大学珠海学院 2020 届本科生毕业论文诚信承诺书本人郑重承诺：本人签名： 日期：年月日套式 PCR 结合基因测序鉴定象科动物物种的研究摘 要为建立一种能准确鉴别象科动物物种的技术方法，本研究针对象科动物基因保守序列，设计并筛选出 2 对特异性引物，建立了以象科动物物种为对象的套式 PCR 结合测序的鉴定方法。并且通过用不同样品及不同浓度样品对该法进行了特异性和灵敏度检验，最后使用本实验中所建立的体系与程序对技术中心的 3 份象牙样品进行了三次重复检测。特异性结果表明该方法可以检测出现代象、猛犸象象牙 DNA 样品，对其他有类似牙、北京理工大学珠海学院 2020 届本科生毕业论文骨构造的动物物种 DNA 样品不扩增；灵敏度试验结果表明套式 PCR 对象科动物物种 DNA样品的检测灵敏度高于荧光 PCR10 倍；3 份临床样品能得到较好的测序结果，测序结果对现代象、猛犸象的物种鉴别较为清晰。本研究建立的套式 PCR 方法能对象牙及象牙制品进行有效、准确的鉴定，对国内关于象牙及象牙制品贸易的监管有一定的帮助。关键词：套式 PCR；荧光 PCR；基因测序；象科动物Identification of Elephant Species by Nested PCR and Gene Sequencing北京理工大学珠海学院 2020 届本科生毕业论文AbstractIn order to establish a technical method that can accurately identify elephant species, this study designed and screened two pairs of specific primers for the conserved sequences of elephant species, and established nested PCR combined sequencing for elephant species. Identification method. And by using different samples and samples of different concentrations to test the method for specificity and sensitivity, and finally using the system and procedure established in this experiment, the three ivory samples of the technical center were tested three times repeatedly. After specificity and sensitivity tests and clinical sample testing, the following relevant results were obtained.The specific results s...
