推拿干预对便秘模型大鼠c

推拿干预对便秘模型大鼠 c【摘要】 目的探讨大鼠后肢活动和便秘的关联性及推拿干预对远端结肠 c-kit 表达的影响, 以讨论推拿治疗便秘的作用机制。方法剪除大鼠后肢股骨，建立便秘模型，经推拿治疗后，分别提取各组大鼠结肠远端细胞总蛋白, 采纳免疫蛋白印迹(Western blot)蛋白半定量方法检测各组标本 c-kit 蛋白表达的变化。结果Western blotting 显示便秘模型大鼠结肠远端 c-kit 蛋白表达明显下降；推拿干预后，c-kit 蛋白表达明显增加。结论大鼠后肢活动减少而引发便秘，可使大鼠结肠远端 c-kit 的蛋白表达减少；经推拿干预后，可使便秘大鼠结肠远端 c-kit 的蛋白表达显着提高，提示推拿治疗便秘与 cajal 间质细胞数目变化有关。【关键词】 便秘 c-kit 免疫印迹 推拿 Abstract：ObjectiveTo approach the relativity of constipation with the decrease of leg activity, and the effect of manipulation on the expression of c-kit protein in rat‘s colon with constipation. MethodsModels of constipation were made by cutting both femur of rat. After manipulation was done, expression of c-kit protein was detected by western-blotting. ResultsThe expression of c-kit protein of rat‘s colon was obviously reduced on model group (P＜) . The expression of c-kit protein of rat‘s colon was obviously increased after treating of manipulation (P＜). ConclusionThe expression of c-kit protein of rat‘s colon was obviously reduced by decreasing the activity of hind limb. The expression of c-kit protein of rat‘s colon was obviously increased after treating of manipulation. We can deduce that the changes of numbers of ICC are related to the manipulation in treating constipation. Key words：Constipation； C-kit； Western blotting; Manipulation 长期卧床患者常便秘，骨折的卧床患者也便秘，根据上述观察建立大鼠便秘模型。推拿是治疗便秘的有效方法，但治疗机制讨论报道较少。Cajal 细胞在胃肠运动中的重要作用已被证实，Cajal 细胞的数目和功能的改变将引起胃肠蠕动的异常，c-kit 是卡哈耳氏细胞的标记...