目录.........................................................1......................................................2..................................................3..............................................3.....................................3.................................5.................................7.............................................10....................................10..................................11...........12...................................................12.............................................12...............................................13.......................................14...............................14.....................................14...................................14...........................15.............15...........................................17..........................17.......................................18...................................19...................................21.........................22...........................................25...................................................25...................................................25....................................................26........................................................29抗菌性银纳米粒子-壳聚糖-溶菌酶复合物的设计摘要银纳米粒子被广泛使用于很多领域，尤其是在抗菌领域。本论文研究了银纳米粒子与溶菌酶相结合的抗菌活性。在本实验中，首先制备了不同粒径的银纳米粒子。我们测试了不同粒径的银纳米粒子的紫外-可见吸收光谱；随后通过透射电子显微镜（TEM）和动态光散射仪（DLS）测量不同粒径的银纳米粒子表面形貌特征和粒径尺寸分布；我们将不同浓度的银纳米粒子与大肠杆菌（MG-1655）混合后涂布在琼脂板上，培养一段时间后观察板上的菌落数目以判断银纳米粒子的抗菌活性与浓度的相关关系。为了测量银纳米粒子的细胞毒性，我们使用CCK-8试剂检测了银纳米粒子对细胞活性的影响。最后，我们将溶菌酶和不同浓度的银纳米粒子结合起来，利用涂板法研究两种杀菌物质结合之后的杀菌效果。结果表明，银纳米粒子的抗菌活性和细胞毒性随着浓度的增加而增大。同时结合溶菌酶后的银纳米粒子的抗菌活性要高于单纯的银纳米粒子，溶菌酶能够显著提高银纳米粒子的抗菌活性。关键词：银纳米粒子；抗菌；细胞毒性；溶菌酶Designofantibacterialsilvernanoparticles-chitosan-lysozymecomplexAbstractSilvernanoparticlesarewidelyusedinmanyfields,especiallyinthefieldofantibacterial.Theantibacterialactivityofsilvernanoparticlescombinedwithlysozymewasstudiedinthispaper.Inthisexperiment,firstofall,wepreparedsilvernanoparticleswithdifferentparticlesizes.WetestedtheUV-Visabsorptionspectraofsilvernanoparticleswithdifferentparticlesizes.Subsequently,thesurfacemorphologyandparticlesizedistributionofsilvernanoparticleswithdifferentparticlesizesweremeasuredbytransmissionelectronmicroscopy(TEM)anddynamiclightscattering(DLS).WemixeddifferentconcentrationsofsilvernanoparticleswithE.coli(MG-1655)andcoatedthemonagarplates.Afterincubationforaperiodoftime,observethenumberofcoloniesontheplatetodeterminetherelationshipbetweenantibacterialactivityandconcentrationofsilvernanoparticles.Inordertomeasurethecytotoxicityofsilvernanoparticles,weusedCCK-8reagenttoexamintheeffectofsilvernanoparticlesoncellviability.Atlast,wecombinedlysozymewithdifferentconcentrationsofsilvernanoparticlesandusedthecoatingmethodtostudythebactericidaleffectofthecombinationoftwobactericidalsubstances.Theresultsshowesthattheantibacterialactivi...
