目录摘要..........................................................................................................1第一章绪论...........................................................................................31.1壳聚糖...........................................................................................31.2聚乙二醇.......................................................................................51.3水凝胶...........................................................................................51.4水凝胶在细胞培养中的作用.......................................................7第二章实验步骤...................................................................................82.1实验仪器与药品...........................................................................82.2磺化壳聚糖的合成.......................................................................92.3双键聚乙二醇的制备.................................................................112.4双键磺化壳聚糖的制备.............................................................112.5水凝胶的制备.............................................................................122.6水凝胶细胞培养.........................................................................13第三章结果与讨论...........................................................................163.1红外光谱分析.............................................................................163.1.1壳聚糖及其衍生物红外光谱分析..........................................163.1.2聚乙二醇与双键聚乙二醇红外光谱分析..............................173.2双键聚乙二醇核磁共振光谱分析.............................................183.3壳聚糖及其衍生物X射线光电子能谱分析.............................183.4水凝胶细胞培养结果及分析....................................................20第四章结论.........................................................................................21参考文献.................................................................................................22致谢........................................................................................................24摘要壳聚糖，是一种及其重要的药物输送及组织工程，用天然生物高分子材料。它具有生物相容性、抗菌性和生物可降解性等非常重要的性质。壳聚糖在生物医药领域尤其是药物载体方面具有广阔的应用前景。本文通过在壳聚糖分子式6-O位接入磺酸基团，制备出6-O-磺化壳聚糖。而后通过酸酐水解，在磺化壳聚糖的氨基位置接入C=C双键，制备得到带有双键的磺化壳聚糖。以聚乙二醇为反应物，通过酰氯的醇解反应，在聚乙二醇分子链的端基接入双键基团。再通过适当的引发剂引发，以双键磺化壳聚糖和双键聚乙二醇及其他物质制备磺化水凝胶，并研究此磺化水凝胶对细胞的培养效果。结果显示，该水凝胶材料可在一定程度上支持细胞的生长。关键词：壳聚糖磺化壳聚糖聚乙二醇水凝胶ThePreparationofSulfonatedChitosanHydrogelforCellCultureAbstractChitosan,asakindofimportantbiomaterialinvolvedwoundhealing,drugdelivery,tissueengineeringandnaturalbiopolymermaterialsdelivery,hasmanyimportantapplicationssuchasbiocompatibility,antibacterialpropertyandbiodegradability,especiallyinthefieldofbiomedicine.Inthispaper,6-O-sulfonatedchitosanwassynthetizedbygraftingthesulfonicacidgrouptothe6-Opositionofchitosan.ThentheC=Cdoublebondwasinsertedattheaminopositionofsulfonatedchitosanbyacidanhydridehydrolysistosynthetizethesulfonatedchitosanwithdoublebond.Regardingpolyethyleneglycolast...
