江西理工大学本科毕业设计（论文）题目：香椿中黄酮类物质提取工艺条件的研究摘要本文以香椿为原料，采用碱提酸析法和微波辅助提取法成功地提取了香椿中的黄酮类物质。经HCL—镁粉反应和红外光谱鉴定，提取物为黄酮类物质。另外，利用紫外分光光度法，以芦丁为对照品，NaNO2-Al(NO3)3-NaOH体系为显色剂测定香椿中总黄酮的含量。并利用L9（3^4）正交试验设计，确立了香椿中黄酮的最佳提取条件及其产率。结果表明，最佳提取工艺条件为硼砂浓度0.5％，料液比1：40，提取30min，温度60℃。结论：本试验可为香椿进一步研究和资源开发利用提供一定的科学依据。关键词:香椿；黄酮类物质；提取；测定ABSTRACTInthispaper，flavonidswereextractedfromChinesemahoganysuccessfully，byusingthemethodof“dissolvedinalkaliandseparatedoutinacid”andmicrowave—assistedmethod．Flavonidswereidentifiedbyreactingwithmagnesium-hydrochloricacidandIRcharacter-ization．Inaddition，alltheflavonidsinChinesemahoganyweredeterminedbyUV-spectrophotometrywithrutinasthestandard，thequantitiesofflavonoidscomponentesweremeasuredwithat360nm.AnduseL9（3^4）orthogonaltestdesign.Resultshowedthattheoptimumextractingconditionsweredeterminedwith0.5%(M／V)Na2B4O7.10H2Oasextractorwiththeratioofmaterialtoliquidat1：40，andextractingfor30min／timeandtheseparationtemperatureis50℃：ThisstudyprovidedscientificbasisforChinesemahogany，developmentandutilizationofgardenia.Keywords：Chinesemahogany；Flavonids；Extraction；Determination目录前言7第一章文献综述..........................................................................81.1香椿的概况.......................................81.2香椿的研究进展...................................81.2.1香椿的产地和品种..............................81.2.2香椿的化学成分研究............................81.2.3药理学研究...................................91.3香椿的开发前景.................................101.4黄酮类化合物的概况.............................101.4.1黄酮类化合物分布及其存在形式................101.4.2黄酮类化合物简介............................111.4.3黄酮类化合物的性质..........................111.5黄酮类化合物研究的意义.........................111.6黄酮类化合物的研究进展.........................121.6.1黄酮类化合物结构及其分类....................121.6.2黄酮类化合物的生理功能与人类健康............121.6.3黄酮类化合物的提取工艺......................151.6.4黄酮类化合物的定性与定量检测...............161.7黄酮类化合物的开发与应用.......................171.7.1市场前景....................................171.7.2黄酮资源开发................................18第二章实验部分........................................................................192.1实验原料与仪器.................................192.1.1原料........................................192.1.2试剂........................................192.1.3仪器........................................192.2黄酮含量测定的原理和方法.......................192.2.1测定原理....................................192.2.2测定方法....................................192.2.3香椿中黄酮的单因素提取试验..................212.2.4确定香椿中黄酮最佳提取工艺条件的正交试验....212.2.5黄酮的鉴定..................................232.2.6香椿的总黄酮的测定..........................25第三章结果与讨论....................................................................263.1重现性实验.....................................263.2测定波长的选择.................................263.3最佳提取条件的选择.............................273.3.1正...
