氧化亚铁硫杆菌的分离及生物学特性研究摘要氧化亚铁硫杆菌(Thiobacillusferrooxidans,简称T.f)属革兰氏阴性无机化能自养菌、嗜酸、专性好氧,靠氧化基质中的Fe2+为Fe3+和低价态硫为硫酸根而获取能量。它广泛存在于土壤海水、淡水、垃圾、硫磺泉和沉积硫内,尤以金属硫化矿和煤矿等酸性矿坑水中最为常见。因此，本论文从T.f菌的分离纯化出发，进行了T.f菌的生理生化及其生物活性的研究。本文从浸矿溶液中分离得到的氧化亚铁硫杆菌进行研究，结果表明硫酸亚铁相对于硫来说是一种速效能源，单质硫是长效能源，并且能提供比亚铁更高的能量。关键词：氧化亚铁硫杆菌，微生物冶金，生物浸矿，分离纯化IsolationofThiobacillusandbiologicalcharacteristicsAbstractFerrooxidans(Thiobacillusferrooxidans,calledT.f),aninorganicchemoautotrophGramnegativebacteria,acidophilus,specificaerobic,relyingonsubstrateoxidationinFe3+andFe2+forthelowstateofsulfurtosulfuricacidrootsandobtainenergy，whichiswidelyfoundinsoilwater,freshwater,waste,anddepositionofsulfurinsulfursprings,especiallyinsulfidemineralssuchasacidminewaterandcoalthemostcommon.Therefore,thispaperfromtheT.fpurificationandidentificationofbacteriainconductingthephysiologicalstrainAfitsbiologicalactivity.Thisseparationfromtheleachingsolutionobtainedferrooxidansstudyresultsshowthatcomparedwithferroussulfatesulfurisakindofquickenergy,sulfurisalong-lastingenergy,andcanprovidemoreenergythantheferrous.Keywords:Thiobacillusferrooxidans,microbioleaehin,Biologicalleaching,isolation目录摘要.................................................IAbstract............................................II一、前言..........................................11.1生物冶金的概念..............................................................................11.2碲矿的生物冶金.............................................................................21.3氧化亚铁硫杆菌的作用..................................................................31.4本实验研究内容..............................................................................3二、实验部分.........................................52.1实验材料与设备..............................................................................52.1.1菌种采集....................................................................................52.1.2实验试剂....................................................................................52.1.3实验仪器....................................................................................52.1.4培养基........................................................................................62.2研究方法..........................................................................................72.2.1菌种富集培养............................................................................72.2.2分离纯化....................................................................................72.2.3单层平板的制作........................................................................82.2.4双层平板的制作........................................................................92.2.5革兰氏染色................................................................................92.2.6pH测定.....................................................................................92.2.7细菌计数方法............................................................................92.4生物特性研究................................................................................112.4...
