产黑色素菌株的培养条件优化 生物化工专业VIP免费

摘要黑色素发展于近现代工业，随着科技日新月异的发展，其在我们生活中也有着十分重要的作用，获得黑色素生产菌株最优培养条件也意味着可以减少黑色素的生产成本。（研究意义）研究的内容和结果本实验采用采用单因子实验方法，对实验室现有黑色素生产菌株进行培养条件优化，考察温度、转速、装液量、初始pH值等条件对黑色素产量的影响，依此得到可以培养出黑色素的最有利条件：培养温度28℃、装液量30%、初始pH=7.8、转速200r/min。在最优培养条件下产黑色素菌株（菌名）的菌株干重为xxxx,黑色素产量为xxx。相比于基本培养条件提升了160%。关键词：链霉菌、黑色素、发酵、培养条件优化IAbstractMelaninplaysaveryimportantroleinbothmodernindustryandourdailylife.Obtainingtheoptimalcultureconditionsofmelaninproducingstrainsalsomeansreducingtheproductioncostofmelanin.Inthisexperiment,singlefactorexperimentmethodwasusedtooptimizethecultureconditionsofmelaninproducingstrainsinthelaboratory.Theeffectsoftemperature,rotationspeed,liquidvolumeandinitialpHvalueonmelaninproductionwereinvestigated,andtheoptimalcultureconditionsformelaninproductionwereobtained.Thefermentationcurvesofmelaninproducingstrainsbeforeandafteroptimizationwerecompared.Theresultsshowedthattheoptimalcultureconditionswere28,30%liquidvolume,initialpH=7.8and200r/min.Undertheoptimalculture℃conditions,thedryweightandmelaninproductionofmelaninproducingstrainswerecompared.Comparedwiththeinitialgrowthcurve.Themelaninyieldwas1.1g/l,whichwas160%higherthantheinitialculturecondition.Keyword:Melanin;High-producingstrain;Productioncosts;Fermentation;StreptomycesstrainII目录摘要.................................................................IAbstract...............................................................II引言..................................................................12材料和方法............................................................41.1材料................................................................41.1.实验所用菌株........................................................41.1.2培养基..........................................................41.1.2.1高氏1号/种子培养基（g·L-1）[11]..............................41.1.2.1发酵培养基（g·L-1）Tyr发酵培养基[4]..........................41.2仪器................................................................42.2方法................................................................52.1培养方法............................................................52.2产黑色素菌株生长曲线的绘制..........................................52.3黑色素纯化..........................................................52.4黑色素产量的测定（标准曲线）........................................53结果与讨论............................................................73.1菌株活化............................................................73.2黑色素测定..........................................................73.3.1标准曲线........................................................73.4黑色素菌株（菌名）的生长曲线绘制....................................7结论...................................................................10参考文献...............................................................13III引言1.1黑色素概述发源在动植物和微生物中的黑色素属于天然色素，它广泛存在于自然界中，属于非人造色素家族。黑色素在一般的情况下不会溶解在酸和绝大部分可购买到的有机溶剂中，当然他也不溶于水【1】。黑色素之所以能够呈现出黑色，主要缘由在于它能够吸收一切波长的可见...