细胞工程实验指导目录实验室安全守则.............................................................................................................................................3实验操作须知.................................................................................................................................................5实验一培养基母液的制备.........................................................................................................................8实验二培养基的配制与灭菌...................................................................................................................12实验三培养材料灭菌和接种...................................................................................................................16实验四愈伤组织的诱导与增殖...............................................................................................................19实验五愈伤组织的分化...........................................................................................................................21实验六植物细胞悬浮培养与同步化.......................................................................................................22实验七细胞微室培养...............................................................................................................................23实验八原生质体培养...............................................................................................................................24实验九烟草原生质体融合.......................................................................................................................26实验十烟草叶片组织培养.......................................................................................................................28实验十一月季组织培养.............................................................................................................................29实验十二菊花组织培养与快速繁殖.......................................................................................................30实验十三马铃薯茎尖脱毒.......................................................................................................................32实验十四马铃薯试管微薯的诱导.........................................................................................................33实验十五小麦幼胚培养...........................................................................................................................34实验十六小麦成熟胚培养.......................................................................................................................36实验十七小麦花药培养...........................................................................................................................38实验十八生物反应器中芹菜体胚发生的大规模培养.........................................................................39实验十九植物细胞的生长计量技术.......................................................................................................41实验二十烟草遗传转化实验.....................................................................................................................45实验二十一细胞原代培养.......................................................................................................................46实验二十二细胞传代培养...................
