血细胞染色方法的探讨 VIP专享VIP免费

长沙医学院毕业设计(论文)课题名称血细胞染色方法的探讨学生姓名张济真学号院(系)、年级专业医学检验系13级检验一班指导教师黄秋炬位松华职称助教主任技师2017年04月30日目录中文摘要....................................................................................................I英文摘要..........................................................................................II前言...................................................................................................11材料与方法....................................................................................21.1实验材料.............................................2主要仪器................................................2试剂及药品..............................................2主要试剂的配制...........................................22实验方法................................................................................................32.1血涂片的制作.........................................32.2Giemsa染色..........................................32.3Wright染色..........................................42.4Giemsa-Wright染色...................................42.5注意事项.............................................43结果..............................................................................................53.1Giemsa染色..........................................5Wright染色.............................................53.3Giemsa-Wright染色...................................54探究不同条件下三种染色方法的效果........................................54.1不同染色条件下Giemsa的染色效果......................64.2不同条件下Wright染液的染色效果......................64.3不同条件下Giemsa-Wright混合染液染色效果.............65讨论..............................................................................................75.1Giemsa氏染色法......................................75.2Wright氏染色法......................................75.3Wright-Giemsa混合法.................................8结论.................................................................................................11参考文献.........................................................................................13致谢15摘要目的：本论文用不同的染色方法对血涂片进行染色，观察血细胞的形态，比较对血细胞的不同辨别结果。方法：我们分布通过Giemsa染色法、Wright染色法和Giemsa-Wright混合液染色3种染色方法对血涂片进行染色并对血细胞的形态学进行观察。结果：在染色时间上Giemsa染液染色时间最长、Wright染液染色时间最短、Giemsa-Wright混合液染色时间介于两者之间，Giemsa染液的细胞核染色效果明显，细胞质界限清晰，但细胞质中的颗粒染色效果较差；Wright染液染对细胞核和细胞质中的颗粒染色效果较好，细胞质的界限不清楚；Giemsa-Wright混合染色结果结合了前两种方法的优点，细胞核和细胞质中的颗粒染色效果明显并且细胞界限清晰。结论：Giemsa染色法、Wright染色法和Giemsa-Wright混合染色三种染色方法均能有效的协助甄别血细胞，Giemsa-Wright混合染色方法相较于前两种方法染色效果更为明显，值得广泛应用。关键词：血细胞；血涂片；Giemsa染色法；Wright染色法；Giemsa-Wright混合染色法；细胞形态学ABSTRACTObjective:Inthispaper,differentstainingmethodswereusedtostainthebloodsmears,observethemorphologyofbloodcells,andcomparethedifferentresultsofbloodcells.Methods:ThebloodsmearswerestainedbyGiemsastaining,WrightstainingandGiemsa-Wrightstaining.Themorphologicalchangeswereobserved.Results:TheGiemsa-Wrightmixturehadtheshorte...