I多壁碳纳米管表面分子印迹聚合物的制备及电化学表征摘要本文以功能化的多壁碳纳米管为载体，以己烯雌酚(DES)为模板分子，丙烯酰胺(AM)为功能单体，EDMA为交联剂，过硫酸铵为引发剂，在多壁碳纳米管表面制备己烯雌酚分子印迹聚合物，将其滴涂在王皮石炭电极表面，构建分子印迹电化学传感器。采用差分脉冲伏安法来优化检测的条件，结果表明，具有分子印迹的电化学传感器具有识别目标分子己烯雌酚的特定能力，并且结合可以快速达到平衡。在己烯雌酚及其结构类似物同时存在时，该传感器对己烯雌酚具有良好的选择特异性。传感器峰电流Ip在己烯雌酚浓度(C)为1×10-9~1×10-6mol/L范围内有良好的线性关系，线性方程为：Ip=6.1744lnC+34.7995，相关系数0.9829，检出限为6.63×10-10mol/L。在实际应用中，该传感器在实际样品中回收率为96.8%~98.8%。关键词：分子印迹；电化学传感器；表面分子印迹法；多壁碳纳米管；己烯雌酚；2AbstractInthispaper,carboxylatedmulti-walledcarbonnanotubeswereusedascarrier,diethylstilbestrol(DES)astemplatemolecule,acrylamide(AM)asfunctionalmonomer,EDMAascross-linkingagent,persulfate.Appliedontothesurfaceofglassycarbonelectrodetoconstructamolecularlyimprintedelectrochemicalsensor.Theresultsshowedthatthemolecularlyimprintedelectrochemicalsensorhasspecificrecognitionabilityforthetargetmoleculediethylstilbestrol,anditsbindingcanquicklyreachequilibrium.Thesensorhasgoodselectionspecificityfordiethylstilbestrolwhendiethylstilbestrolanditsstructuralanaloguesarepresentsimultaneously.Thepeakcurrentofthesensorhasagoodlinearrelationshipintheconcentrationofdiethylstilbestrol(C)of1×10-9~1×10-6mol/L.Thelinearequationis:Ip=6.1744lnC+34.7995Thecorrelationcoefficientis0.9829,andthedetectionlimitis6.63×10-10mol/L.Inpracticalapplications,therecoveryrateofthesensorintheactualsampleis96.8%~98.8%.Keywords：molecularimprinting;electrochemicalsensor;surfacemolecularimprinting;multi-walledcarbonnanotubes;diethylstilbestrol目3录摘要.........................................................................................................................................IAbstract......................................................................................................................................II第1章绪论...............................................................................................................................11.1分子印迹技术.................................................................................................................11.1.1分子印迹技术简介...................................................................................................11.1.2分子印迹技术原理...................................................................................................11.1.3分子印迹技术的分类...............................................................................................21.1.4分子印迹聚合物合成方法的选择...........................................................................21.1.5分子印迹聚合物制备条件的选择...........................................................................31.2分子印迹技术的应用.....................................................................................................41.2.1色谱分离...................................................................................................................41.2.2固相萃取剂...............................................................................................................41.2.3传感器........................
