H3K27me3在胃癌中的表达及其临床意义_高福利VIP免费

wcjd@wjgnet.com世界华人消化杂志2011年12月18日;19(35):3597-3602ISSN1009-3079(print)ISSN2219-2859(online)临床研究CLINICALRESEARCHH3K27me3在胃癌中的表达及其临床意义高福利,吕瑛,曹俊,邹晓平www.wjgnet.com®■背景资料组蛋白修饰在肿瘤发生、发展过程中起着重要的作用.近年研究发现多种组蛋白修饰在恶性肿瘤中发生改变,且这种变化还有重要的临床应用价值,如作为判断患者预后的指标、肿瘤早期诊断的标记物、判断对某些化疗药物的治疗反应.高福利,吕瑛,曹俊,邹晓平,南京大学医学院附属鼓楼医院消化内科江苏省南京市210008高福利,硕士,主要从事胃癌的表观遗传学研究.南京市医学科技发展基金资助项目,No.YKK08066作者贡献分布:此课题由高福利、吕瑛、曹俊及邹晓平设计;研究过程由高福利完成;研究所用新试剂及分析工具由吕瑛与邹晓平提供;数据分析由高福利完成;本论文写作由高福利与邹晓平完成.通讯作者:邹晓平,教授,210008,江苏省南京市中山路321号,南京大学医学院附属鼓楼医院消化内科.zouxiaoping795@hotmail.com电话:025-83106666-20602收稿日期:2011-09-28修回日期:2011-11-12接受日期:2011-12-07在线出版日期:2011-12-18SignificanceofH3K27me3expressioningastriccancerFu-LiGao,YingLv,JunCao,Xiao-PingZouFu-LiGao,YingLv,JunCao,Xiao-PingZou,DepartmentofGastroenterology,theAffiliatedDrumTowerHospitalofMedicalSchoolofNanjingUniversity,Nanjing210008,JiangsuProvince,ChinaSupportedby:theNanjingMedicalTechnologyDevelop-mentProject,No.YKK08066Correspondenceto:Xiao-PingZou,Professor,Depart-mentofGastroenterology,theAffiliatedDrumTowerHospitalofMedicalSchoolofNanjingUniversity,Nanjing210008,JiangsuProvince,China.zouxiaoping795@hotmail.comReceived:2011-09-28Revised:2011-11-12Accepted:2011-12-07Publishedonline:2011-12-18AbstractAIM:Toinvestigatetheexpressionoftrimethyl-ationatlysine27ofhistoneH3(H3K27me3)ingastriccancertissueandcelllinesandtoevalu-ateitscorrelationwithclinicopathologicalpa-rametersofgastriccarcinoma.METHODS:TheproteinexpressionofH3K27me3wasdetectedbyWesternblotingastriccancerlinesSGC7901,BGC823,AGSandnormalgastricmucosalepithelialcelllineGES-1.Immunohis-tochemistry(IHC)wasutilizedtoexaminetheproteinexpressionofH3K27me3in61gastriccancerspecimensand20normalgastricepithe-lialspecimens.RESULTS:H3K27me3expressionsignificantlyincreasedingastriccancerlines(SGC7901,BGC823,andAGS)comparedtonormalgastricmucosalepithelialcelllineGES-1.ThepositiveratesofH3K27me3proteinexpressioningas-triccancerwas80.3%.TheexpressionlevelsofH3K27me3weresignificantlyassociatedwithtumorsize,depthofinvasion,lymphnodeme-tastasis,vascularinvasion,clinicalstage,andTstaging(P=0.049,0.030,0.034,0.025,0.003,and0.031,respectively),buthadnocorrelationwithpatient’sage,sex,tumorlocation,tumordiffer-entiationdegree,ornerveinvasion.CONCLUSION:HighexpressionofH3K27me3correlatescloselywithtumorinvasionandme-tastasisingastriccancerandmaybeanimpor-tantprognosticfactorinpatientswithgastriccancer.KeyWords:Histonemodification;H3K27me3;Gas-triccancerGaoFL,LvY,CaoJ,ZouXP.SignificanceofH3K27me3expressioningastriccancer.ShijieHuarenXiaohuaZazhi2011;19(35):3597-3602摘要目的:研究H3K27me3在胃癌细胞和组织中的表达,并分析与临床病理因素的关系,探讨H3K27me3在胃癌发生发展中的作用和意义.方法:应用Westernblot方法检测胃癌细胞系SGC7901、BGC823、AGS和正常胃黏膜上皮细胞GES-1中H3K27me3的表达;免疫组织化学方法检测61例胃癌组织及20例正常胃黏膜组织中H3K27me3的表达.结果:与正常胃黏膜细胞GES-1相比,H3K27me3在胃癌细胞SGC7901、BGC823、AGS中高表达;H3K27me3在胃癌组织中阳性表达率为80.3%,并与肿瘤大小、浸润深度、淋巴结转移、血管侵犯...