Ju1．200622(4)419—422神经解剖学杂志(ChineseJournalofNeuroanatomy)Nestin在高温致畸神经管的表达及多种维生素和微量元素对神经管畸形的保护作用①孙秀宁管英俊张皓云岳炳德于丽李锋杰(潍坊医学院组织胚胎学教研室，潍坊261042)摘要本研究旨在探讨高温致神经管畸形(NTD)中巢蛋白(nestin)的表达规律及多种维生素和微量元素对NTD的保护作用。孕鼠随机分为实验组和对照组。实验组又进一步分为3组，A组：孕前第8d开始灌服多种维生素和微量元素；B组：孕后第1d开始灌服；C组：不灌服。孕鼠分别于孕第8d高温处理，对照组不灌服多种维生素和微量元素，也不进行高温处理。对以上各组胎鼠进行形态学观察，计算神经管畸形发生率；利用免疫荧光染色观察nestin在胚胎不同发育时期的神经上皮细胞中的表达规律。结果显示，在实验组中，C组神经管畸形发，E率最高，为77．5％；各组鼠胚神经上皮细胞胞质中nestin呈阳性染色．实验C组的染色明显弱于其它组；不同发育阶段各组染色强度不同。结果提示，nestin低表达是神经管畸形发生的重要因素，多种维生素和微量元素对高温致神经管畸形有保护作用。关键词神经管畸形巢蛋白维生素微量元素THEEXPRESSIoNoFNESTDANDTHERoLESoFVrrAMDSANDMICRoELEMENTSlNNEURALTUBEDEFECT耵DUCEDBYHYPERTHERMIASunXiuning，GuanYin~un，ZhangHaoyun，YueBingde，YuLi，Li~ngiie(DepartmentofHistologyandEmb~ology，MedicalCollegeofWeifang，Weifang261042)AbstractTheaimofthepresentstudyistoexploretheexpressionofnestinintheneuroepitheliaofembry0swithneuraltubedefect(NTD)inducedbyhyperthermia，andtoobservetheprotectiverolesofvitaminsandmicroelementsinthedevelopmentofNTD．Theani-malsweredividedrandomlyintoexperimentalgroupandcontrolgroup．Experimentalgroupwassubdividedinto3subgroups、IngroupA．animalsweregivenvitaminsandmicroelementsonday8beforepregnancy；IngroupB，vitaminsandmicroelementsweregivenonday1ofpregnancy；AnimalsingroupCwerenotreceivedvitaminsandmicroelementsadministration．Animalsinabove3experimentalgroupswereexposedtohyperthermiaonday8ofgestation．Animalsinthecontrolgroupwerenotreceivedvitaminsandmicroelementsadministrationaswellashyperthemiatreatment．Themorphologicalchangesofhamster~tuseswereobservedstainingwssusedtoex-aminetheexpressionofnestinintheemb~onicneuroepithelia．TheresultsshowedthattheincidencerateofNTDinexperimentalCgroupwasthehighest．about77．5％．Thenestin-positivecellswereobservedintheneuroepitheliaofeachgroup．Thestainingintensityinex-perimcntalgroupCwasmuchweakerthanthatinothergroups．Theintensityofnestinstainingvariedatdiferentemb~onicstagesineachgroup．TheseresultsindicatethatdecreasedexpressionofnestinmaybeinvolvedinthemechanismsofNTDinducedbyhyperthermia，dur-ingwhichvitaminsandmicroelementsmayplayprotectiveroles．Keywordsneuraltubedefect，nestin，vitamin，microelement高温可引起人类和动物多种畸形，是常见的一种环境致畸因子，并且在自然环境、劳动环境中难以①山东省自然科学基金青年基金(No．Q99C12)资助项目通讯作者电话：0536．8707080E-mail：sxn321@163．corn维普资讯http://www.cqvip.com-啦电【g蚌山,qi性4敛畸仔f帆HmJ究渐rJ．’i'l!j，探l-ili敛均H【J北渐fi1营呲{⋯ⅥIl【¨·．N'II1)nfI设{血J垃匀1iJJ『Ⅲ圮们ll之仁蛤』-川』撤书畸肚ild过饱蓰托水舰啭*l正III¨j，卜腑小⋯i发川H；_¨flIT坪¨监细胞『-J均J上J地|i，连gr}11：It似fl}L寮IN'I’Il发I'／~j川圳FIl帕!埘竹】fj提f』lfIf、材料和方法l实验动物殛标本制备峨杷强i雌此地I20i【(北1物圳I硼咒挺小)．rfi《fO0±I_】)，．砒州荆rl1．~译．悦7～9时·蚍l：Ifi-咤j』暾}．j进涂片r阿r苹ItJ帕挑儿讣骑^、llC刈I_f{i^川JH—l潍Ⅲ芷多种lfI书垃i_；_2砒L采(拖、lk．c一珐．湘也啦l鲥仃tJ．批0305103)：IIIItlIJl{物剃{：均250}L_HlitJ...
