鸭坦布苏病毒病防治关键技术研究及应用Keytechnologyresearchandapplicationofducktembusuvirusdiseasecontrol目录摘要......................................................................................................................................VAbstract................................................................................................................................VI缩略词表.................................................................................................................................I第一章综述...........................................................................................................................21鸭坦布苏病毒病.........................................................................................................22病原学.........................................................................................................................22.1生物学分类..............................................................................................................22.2形态结构及理化培养特性......................................................................................23鸭坦布苏病毒病的主要流行病学特征.....................................................................24江西省蛋鸭场鸭坦布苏病毒病流行概况.................................................................35鸭坦布苏病毒诊断方法.............................................................................................45.1临床诊断.................................................................................................................45.2实验室诊断方法......................................................................................................45.2.1病原检测.............................................................................................45.2.2免疫组化方法.....................................................................................55.2.3间接免疫突光试验.............................................................................55.2.4酶联免疫吸附试验.............................................................................6第二章研究的目的及意义..................................................................................................7第三章材料与方法..............................................................................................................81材料.............................................................................................................................81.1实验动物及病料来源..............................................................................................81.2主要试剂..................................................................................................................81.3菌株与细胞..............................................................................................................81.4常用试剂及缓冲液配制..........................................................................................81.5主要仪器..................................................................................................................81.6分析软件及说明......................................................................................................92病原分离鉴定实验.................................................................................................
