下载后可任意编辑信号放大时间分辨荧光分析技术的讨论进展及应用【摘要】 信号放大时间分辨荧光分析技术是一种新型的超微量的标记分析技术。它是以生物信号放大为基础，以镧系元素螯合物为标记物，以时间分辨和波长分辨为测量方法，充分利用了酶标记分析和 PCR扩增等分析技术优点的技术。它的独特优点是非同位素和超灵敏度。本综述介绍了TRFA 的特点以及酶放大、二次酶放大、铕纳米颗粒、滚动循环放大、免疫 PCR 和荧光淬灭分析等信号放大时间分辨荧光分析下载后可任意编辑技术的讨论进展及应用。信号放大时间分辨荧光分析技术在生物分析中极其广泛的应用，显示出具有很好的进展前景。 【关键词】 信号放大；时间分辨荧光分析；镧系元素螯合物 Studies and applications of time-resolved fluorescence assay based on signal amplification 【Abstract】 Time-resolved fluorescence assies(TRFA) based on signal amplification are new 下载后可任意编辑ultrasensitive labelling assay basis is biosignal amplification,the labels are lanthanide chelates,and the detection methods are the time-resolved and wavelength-resolved fluorescence assay in the addition,enzyme-labelled assay and PCR amplification et al are used in the several main signal amplification methods and amplifications, for example, enzyme-下载后可任意编辑amplification, two-round enzyme-amplification,europium nanopartical,rolling circle amplification,immuno-PCR and fluorescence quenching assay et al, were introduced in the summary. The unique advantages of nonisotope and ultrasensitivity and extensive applications in bioassay shown good prospects of TRFA based on signal amplification.下载后可任意编辑 【Key words】 signal amplification; time-resolved fluorescence assay; lanthanide chelate 近些年来，非同位素标记分析技术进展迅速。它主要包括：酶标记分析技术、化学发光分析技术、生物发光分析技术和时间分辨荧光分析技术。信号放大时间分辨荧光分析技术克服了其他技术的缺点，具有非同位素、灵敏度高、标记物制备简单、测量快速和分析动态范围宽等优点，下载后可任意编辑成为一...
