精品文档---下载后可任意编辑系统性红斑狼疮患者中泌乳素介导的 JAK/STAT 信号通路及 AG490 的干预讨论的开题报告【摘要】本讨论旨在探讨系统性红斑狼疮（SLE）患者中泌乳素介导的 JAK/STAT 信号通路及 AG490 的干预作用。讨论采纳体外细胞实验和动物实验相结合的方式进行。首先，通过 Western blotting、病理切片等方法检测 SLE 患者外周血单个核细胞（PBMC）中 JAK/STAT 信号通路的活性，以此为基础进一步观察泌乳素对 PBMC 的诱导作用。其次，在小鼠模型中投注泌乳素，观察其对小鼠免疫系统的影响。最后，采纳AG490 对泌乳素介导的 JAK/STAT 信号通路进行干预，并评估其对 SLE的治疗作用。【关键词】系统性红斑狼疮；JAK/STAT 信号通路；泌乳素；AG490【Abstract】The objective of this study is to investigate the role of prolactin-mediated JAK/STAT signaling pathway and AG490 intervention in patients with systemic lupus erythematosus (SLE). The study combines in vitro cell experiments and animal experiments. Firstly, the activity of the JAK/STAT signaling pathway in peripheral blood mononuclear cells (PBMCs) of SLE patients was detected through Western blotting, pathological sections and other methods, and the induction effect of prolactin on PBMCs was further observed on this basis. Secondly, the effect of prolactin on mouse immune system was observed by injecting prolactin into mouse models. Finally, AG490 was used to intervene in the prolactin-mediated JAK/STAT signaling pathway and evaluate its therapeutic effect on SLE.【Keywords】Systemic lupus erythematosus; JAK/STAT signaling pathway; prolactin; AG490
