精品文档---下载后可任意编辑VEGF 基因组织工程联合 ERK 通路干预减轻硬膜外瘢痕形成的讨论的开题报告摘要：硬膜外瘢痕形成是硬膜外血管膜手术的常见并发症之一，严重影响手术效果和患者生活质量。本讨论旨在通过 VEGF 基因组织工程联合ERK 通路干预，减轻硬膜外瘢痕形成，提高手术效果和患者生活质量。讨论将分为以下三个步骤：1）构建慢病毒表达载体；2）体内模型建立及试验组设计：将 Wistar 大鼠分为对比组、单纯 VEGF 组、单纯 ERK组及 VEGF+ERK 组，通过脊髓硬膜外分离手术建立模型，随访记录硬膜外瘢痕形成情况及手术效果；3）实验结果分析：运用 HE 染色、Masson 染色及免疫组织化学染色对硬膜外区域进行形态分析，比较四组动物硬膜外瘢痕形成情况以及组织学差异。估计结果将展示 VEGF 基因组织工程联合 ERK 通路干预在减轻硬膜外瘢痕形成及提高手术效果方面的潜在价值，为硬膜外血管膜手术提供新的治疗方法。关键词：硬膜外瘢痕形成；VEGF 基因组织工程；ERK 通路；慢病毒表达载体；免疫组织化学染色Abstract:Epidural scar formation is a common complication of epidural vascular membrane surgery, which seriously affects the surgical effect and the quality of life of patients. This study aims to reduce epidural scar formation and improve surgical efficacy and quality of life through VEGF gene tissue engineering combined with ERK pathway intervention. The study will be divided into the following three steps: 1) construction of lentiviral expression vector; 2) establishment of in vivo model and experimental group design: Wistar rats will be divided into control, single VEGF, single ERK and VEGF+ERK groups. The model will be established by spinal epidural separation surgery, and the epidural scar formation and surgical effect will be followed up; 3) analysis of experimental results: HE staining, Masson staining and immunohistochemistry staining were used to analyze the morphology of the epidural region, compare the epidural scar formation and histological differences of the four groups of animals. The expected results will show the potential value of VEGF gene tissue engineering combined with ERK pathway intervention in reducing epidural scar formation and improving 精品文档---下载后可任意编辑surgical efficacy, providing new treatment methods for epidural vascular membrane surgery.Keywords: epidural scar formation; VEGF gene tissue engineering; ERK pathway; lentiviral expression vector; immunohistochemistry staining.
