精品文档---下载后可任意编辑二倍体 OT 百合 2n 配子产生及杂交后代鉴定的开题报告Title: Identification of hybrid offspring after the production of diploid OT lily 2n gametes and hybridizationIntroduction:In plant breeding, creating new hybrids is often desirable for developing new cultivars with desirable traits. However, for some plants, hybridization is difficult or impossible due to various factors including polyploidy. In lilies, for example, interspecific hybridization is challenging due to differences in ploidy levels between different species. Diploid Oriental Trumpet (OT) lilies (2n=2x=24) have been developed by treating haploid microspores with colchicine, but their use in hybridization to produce new cultivars has been limited. However, recent advances in in vitro culture techniques have made it possible to produce diploid 2n gametes from OT lilies, paving the way for more efficient hybridization.Objectives:The objective of this study is to produce diploid 2n gametes from OT lilies using in vitro culture techniques and to use these gametes to produce interspecific hybrids. The offspring of the hybrids will be identified using molecular markers to evaluate the success of the hybridization process.Methods:Diploid OT lily plants will be propagated in vitro and shoot tips will be used as explants for callus induction. The callus will be induced on Murashige and Skoog (MS) medium supplemented with plant growth regulators. Embryogenic calli will be selected and cultured on a medium with colchicine to induce chromosome doubling. The resulting diploid 2n gametes will be used for hybridization with other lily species. To identify the hybrid offspring, molecular markers such as AFLP (Amplified Fragment Length Polymorphism) and SSR (Simple Sequence Repeat) will be used to analyze DNA extracted from the offspring plants.Expected outcomes:精品文档---下载后可任意编辑By producing diploid 2n gametes from OT lilies and using them for hybridization, we expect to produce new interspecific hybrids that were previously not possible with standard haploid gametes. The use of molecular markers will allow us to confirm the success of the hybridization process and identify the offspring plants. These new hybrids may exhibit desirable traits such as disease resistance, better growth habits, and unique flower characteristics, making them valuable for the floriculture industry.
