动物性食品中磺胺类药物残留elisa方法检测的建立本科学位论文

本科毕业论文动物性食品中磺胺类药物残留 ELISA方法检测的建立Establishment of ELISA Methods and Kits for Rapid detection of Sulfonamides in the food of animals 学 院：兽医学院专 业：动植物检疫姓 名：朱晓彤学 号：121716570指导老师：郝永清职 称：教 授 完成日期：二 Ο 一六年五月摘 要磺胺类药物是一类广谱抗菌药，广泛用于预防、治疗细菌性疾病，还常作为饲料添加剂长期在动物饲养过程中使用。大量实验证明长期使用磺胺类药物不仅使人和动物产生耐药性，还会引起人过敏性反应，有致癌、致畸、致突变的作用。磺胺类药物的不合理使用已经严重危害人类健康。因此，讨论出一种操作简便、快速、灵敏的磺胺类药物残留检测方法，对于畜产品的安全具有实际意义。酶联免疫吸附法(ELISA)作为一种免疫学检测技术不仅具有上述优点，而且准确性好、特异性强、检测成本低，还可用于大批量样品的检测。本文是对北京维德维康生物技术有限公司兽药残留 ELISA 检测方法建立及其效果进行评价和讨论。关键词: 动物性食品 磺胺类药物 残留 检测Abstract Sulfonamides are antibiotics which have wide antimicrobial spectrum. They are used to prevent and cure bacterial infections.SAS are usually added into forage to feed animals. But sulfonamides can cause anaphylactic reaction and carcinogenic mutagenic teratogenic. people. sulfonamide residues for its irrational use can affect people health .Therefore, it is significant to study a method of sulfonamides detection in animal product security. Enzyme linked Immunosorbent assay(ELISA) is a convenient, fast, sensitive method,which has accurate and strong specificity . The method costs lowly and can detect a large number of samples. This article is based upon establishment and effect analysis and discussion of the ELISA for detection of veterinary drug residues by Beijing WDWK Biotechnology Co., Ltd.Key words: Animal food; Sulfonamides; Residue; Detection目 录1.引言...............................................................................................