响应面分析法优化枸杞多糖提取工艺条件VIP专享

响应面分析法优化枸杞多糖提取工艺条件【摘要】 目的利用响应面分析法对枸杞多糖的提取工艺进行优化。方法在单因素实验基础上选取实验因素与水平，根据中心组合实验设计原理采纳 3 因素水平的响应面分析法，依据回归分析确定各工艺条件的影响因子，以多糖提取率为响应值作响应面和等高线。结果在分析各个因素的显着性和交互作用后，得出枸杞多糖水浸提的最佳工艺条件为：料液比1∶32；浸提温度 84℃；浸提时间；浸提 1次。结论在最佳工艺条件下，枸杞多糖的实际提取率可达%。【关键词】 枸杞 枸杞多糖 提取 响应面分析 Abstract：Objective To optimize the extraction technology of Lycium barbarum polysaccharides by response surface factors and levels were firstly selected by one factor tests. On the basis of the previous results and according to the central composite experimental design principles,the method of Response Surface Method with 3 factors and 3 levels was the factors influencing the technological parameters were determined by means of regression surface and contour were finally graphed with the extraction rate as the response optimum conditions of Lycium barbarum polysaccharides extraction were as follows:the ratio of material to solvent of 1∶32,extraction temperature of 84℃ and extraction time of h,one time yield of Lycium barbarum polysaccharides is % under the optimum conditions. Key words：Lycium barbarum ; Lycium barbarum polysaccharides ; Extraction; Response surface methodology 枸杞是多年生灌木，花紫、果红、枝繁叶茂，具有滋补肝肾、益精明目的功效［1］。枸杞子是我国传统中药，具有很高的药食两用价值。近年来，枸杞作为一种新兴的保健型食用植物在我国宁夏、新疆等地区进行了大面积种植，种植面积逐年增加。众多的讨论表明，枸杞多糖具有保肝、降血糖、降血脂、抗肿瘤、抗衰老等多种生理功效［2～7］。因此，提取枸杞多糖对开发和利用枸杞资源具有重要意义。 响应面分析(Response Surface Methodology)法系采纳多元二次回归方法作为函数估量的工具，将多因子实验中因素与指标的相互关系用多...