多西紫杉醇诱导肿瘤细胞凋亡机制的研究目录摘要.........................................................................................................................................IAbstract..................................................................................................................................II1.1材料和试剂...............................................................................................................21.2仪器...........................................................................................................................22.1细胞培养...................................................................................................................32.2细胞的维持和培养...................................................................................................42.2.1细胞的复苏.....................................................................................................42.2.2A549细胞更换新的培养液.........................................................................42.2.3A549细胞的传代............................................................................................52.3分组给药...................................................................................................................52.4MTT溶液的配置与检测..........................................................................................52.4.1配制.................................................................................................................6第3章实验计算以及结果...................................................................................................73.1计算IC50..................................................................................................................73.2实验结果...................................................................................................................7参考文献..............................................................................................................................10摘要目的观察多西紫杉醇诱导hepg2PC3MD-231A549四种肿瘤细胞凋零并对其作用机制进行探讨。方法培养4种细胞，取对数生长期细胞分为五组，空白对照组、模型组、蜂胶黄酮高、中、低剂量组，剂量分别为200mg/L、100mg/L、50mg/L.药物预处理2h后，做MTT检测。通过实验数据计算IC50.结果各组细胞IC50值结论多西紫杉醇对肿瘤细胞有诱导凋亡作用关键词多西紫杉醇；hepg2；A549；PC3；M-231；；细胞凋亡AbstractStudyontheeffectofdocetaxelontumorcellproliferationinhibitionandintumorchemotherapy,takethecellcount,morphologicobservation,flowcytometrymethodforthedetectionandobservationrespectivelyandcombinedwithdocetaxelonproliferationofhumantumorcelllinesinnudemicebearinghumantumorinhibition;toestablishanimalmodel,totreatseparatelyandcombinedwithdocetaxel,andblankcontrol.Observationofgrowthandpathologicalcharacteristicsofthetumorbody.ThroughtheexperimentsofMTTdetectiontestofdocetaxelontumorcellproliferationinhibition.Usingdifferentconcentrationsoftumorcellsuppressiontest,theinfluenceoftheconcentrationonthesurvivaloftumorcells.Theexperimentisdividedintotwoparts,respectively,andMTTdetectionofcellculture.Keywords:DocetaxelStemcellsResearchApplication前言紫杉类药物属于红豆杉的树皮或针叶中提取或半合成的新抗肿瘤药物，作用在微管以及微管蛋白系统，紫杉类药物与其他植物碱不同，紫杉类药物通过促进微管双聚体装配成微管通过防止去多聚化过程而使...
