目录摘要............................................................................................................1Abstract....................................................................................................2第一章绪论..............................................................................................41.1蛋白质及其功能............................................................................41.2蛋白质的活性调控........................................................................41.3纳米颗粒........................................................................................51.4刺激响应性聚合物........................................................................71.4.1pH响应性聚合物.................................................................71.4.2温度响应性聚合物...............................................................81.5课题提出........................................................................................81.5.1研究目的及意义...................................................................81.5.2研究内容及方案...................................................................8第二章实验结果与讨论........................................................................102.1试剂与药品..................................................................................102.2药品预处理..................................................................................102.3实验设备.......................................................................................112.4缀合物的合成..............................................................................112.4.1金纳米粒子的合成.............................................................112.4.2生成巯基修饰的PPase.......................................................122.4.3巯基官能化的PMAA的合成............................................122.4.4PNIPAM的RAFT合成......................................................122.4.5吡啶基二硫化物去偶功能化PNIPAM..............................132.4.6AuNP-PPase-PNIPAM缀合物的合成................................132.4.7AuNP-PPase-PNIPAM-PMAA缀合物的制备...................132.4.8AuNP-PPase-PNIPAM-PMAA缀合物的表征...................14第三章实验结论与分析........................................................................153.1PMAA和PNIPAM的RAFT聚合..............................................153.2金纳米粒子制备..........................................................................173.3缀合物的制备与表征..................................................................17第四章结论与展望................................................................................20参考文献..................................................................................................21致谢..........................................................................................................22摘要刺激响应性聚合物在调控蛋白活性、药物释放等生物医用和生物化学领域有重要的应用价值。本文中，我们探究了pH和温度双响应蛋白调控体系。通过可逆加成-断裂链转移（RAFT）聚合聚合合成了聚丙烯酸甲酯(PMAA)和聚异丙基丙烯酰胺(PNIPAM)，前者为pH响应性聚合物，后者为温度响应性聚合物。通过改变投料比和控制聚合条件合成具有多重环境响应性质的刺激响应性聚合物。通过金纳米粒子（AuNP)先与无机焦磷酸酶（PPase）形成AuNP-PPase缀合物，人工合成的刺激响应性聚合物再通过Au-S键接在金纳米...
