III摘要目的：研究三种常见花茶有效成分的提取工艺的优化及其抑菌作用。通过不同超声时间取值的最适时间实验、不同超声功率取值的最适功率实验、不同料液比取值的最佳比例实验、不同提取时间取值的最适时间实验、不同乙醇浓度取值的最佳浓度实验，先进行了单因素实证试验并展开分析，之后又进行正交实验，从而确定三种常见花茶有效成分的最适提取条件；通过测量抑菌圈大小、MIC、MBC值，比较绿原酸和总黄酮提取液这两种不同场景环境下，对大肠杆菌能够发挥的抑菌效果，及对金黄色葡萄球菌起到有效抑菌作用，同时还有枯草芽孢杆菌能够达到的抑菌效果，实验结果表明，针对金银花绿原酸进行提取过程中最适条件具体分别是：超声时间有效取值是15min，料液比有效取值是1:30(g/mL)；超声功能有效取值是180W，提取时间有效取值为60min；与此同时，针对杭白菊总黄酮进行提取过程中最适条件具体分别是：料液比有效取值是1:15(g/mL)；提取温度有效取值是50℃；提取时间有效取值是70min；另外，针对玫瑰花总黄酮进行提取过程中最适条件具体分别是：乙醇浓度有效取值是70%，超声次数有效取值是3次，超声时间有效取值是30min，料液比有效取值是1:25(g/mL)；从实验所得各种数据和结果显示，绿原酸表现显著，另外总黄酮也表现突出，二者的提取液对枯草芽孢杆菌的抑制作用最显著。结论：通过对三种花茶有效成分的提取及抑菌效果研究，为天然抑菌剂的开发和生产应用提供参考。关键词：金银花；杭白菊；玫瑰花；绿原酸；黄酮；提取；抑菌圈；MIC；MBCIVAbstractObjective:Tostudytheextractionprocessoptimizationandbacteriostasisofthreekindsofeffectivecomponentsofscentedtea.Throughdifferentultrasonictime,thedifferentvaluesbetweenthemosttimelyexperimentsofultrasonicpowervalueoftheoptimalpowerexperiment,differentsolid-liquidratiovalueasthenumberofthebestproportionoftheexperiment,differentextractingtimebetweenthemosttimelyexperiment,theoptimalconcentrationofethanolconcentration,experiment,firsthascarriedontheempiricaltestandanalysis,singlefactorandorthogonalexperiments,afterTodeterminetheoptimalextractionconditionsofthreecommonactivecomponentsofscentedtea;Bymeasuringthebacteriostaticcirclesize,MICandMBCvalues,thebacteriostaticeffectsofchlorogenicacidandtotalflavonoidextractsonEscherichiacoli,Staphylococcusaureus,andBacillussubtiliswerecomparedundertwodifferentenvironments.Theexperimentalresultsshowedthat,TheoptimalconditionsfortheextractionofchlorogenicacidfromLoniceraejaponicaewereasfollows:theeffectivevalueofultrasonictimewas15min,andtheeffectivevalueofsolid-liquidratiowas1:30(g/mL);Theeffectivevalueofultrasonicfunctionwas180W,andtheeffectivevalueofextractiontimewas60min.Atthesametime,theoptimalconditionsfortheextractionoftotalflavonoidsfromChrysanthemumsinensiswereasfollows:theeffectivevalueofsolid-liquidratiowas1:15(g/mL);Theeffectivevalueofextractiontemperatureis50℃.Theeffectivevalueofextractiontimewas70min.Inaddition,theoptimalconditionsfortheextractionoftotalflavonoidsfromrosewereasfollows:theeffectivevalueofethanolconcentrationwas70%,theeffectivevalueofultrasonicfrequencywas3times,theeffectivevalueofultrasonictimewas30min,andtheeffectivevalueofsolid-liquidratiowas1:25(g/mL).Variousdataandresultsobtainedfromtheexperimentshowedthatchlorogenicacidandtotalflavonoidswereprominent,andtheirextractshadthemostVsignificantinhibitoryeffectonBacillussubtilis.Conclusion:Theextractionandantibacterialeffectofthreeactivecomponentsofscentedteawerestudiedtoprovidereferenceforthedevelopment,productionandapplicationofnaturalantibacterialagents.Keywords:LonicerajaponicaThurb;Chrysanthemummorifolium;RosarugosaThunb;Chlorog...
