成都理工大学毕业设计氧化亚铁硫杆菌的分离及生物学特性研究作者姓名：冯雷专业：生物工程指导老师：谢鸿观摘要氧化亚铁硫杆菌(Thiobacillusferrooxidans,简称T.f)属革兰氏阴性无机化能自养菌、嗜酸、专性好氧,靠氧化基质中的Fe2+为Fe3+和低价态硫为硫酸根而获取能量。它广泛存在于土壤海水、淡水、垃圾、硫磺泉和沉积硫内,尤以金属硫化矿和煤矿等酸性矿坑水中最为常见。因此，本论文从T.f菌的分离纯化出发，进行了T.f菌的生理生化及其生物活性的研究。本文从浸矿溶液中分离得到的氧化亚铁硫杆菌进行研究，结果表明硫酸亚铁相对于硫来说是一种速效能源，单质硫是长效能源，并且能提供比亚铁更高的能量。关键词：氧化亚铁硫杆菌，微生物冶金，生物浸矿，分离纯化I成都理工大学毕业设计IsolationofThiobacillusandbiologicalcharacteristicsName:FengLeiMajor:BioengineeringSupervisor:XieHongguanAbstractFerrooxidans(Thiobacillusferrooxidans,calledT.f),aninorganicchemoautotrophGramnegativebacteria,acidophilus,specificaerobic,relyingonsubstrateoxidationinFe3+andFe2+forthelowstateofsulfurtosulfuricacidrootsandobtainenergy，whichiswidelyfoundinsoilwater,freshwater,waste,anddepositionofsulfurinsulfursprings,especiallyinsulfidemineralssuchasacidminewaterandcoalthemostcommon.Therefore,thispaperfromtheT.fpurificationandidentificationofbacteriainconductingthephysiologicalstrainAfitsbiologicalactivity.Thisseparationfromtheleachingsolutionobtainedferrooxidansstudyresultsshowthatcomparedwithferroussulfatesulfurisakindofquickenergy,sulfurisalong-lastingenergy,andcanprovidemoreenergythantheferrous.Keywords:Thiobacillusferrooxidans,microbioleaehin,Biologicalleaching,isolationII成都理工大学毕业设计目录摘要.....................................IAbstract................................II一、前言............................11.1生物冶金的概念...........................................................................................................................11.2碲矿的生物冶金..........................................................................................................................21.3氧化亚铁硫杆菌的作用...............................................................................................................31.4本实验研究内容...........................................................................................................................3二、实验部分.............................52.1实验材料与设备...........................................................................................................................52.1.1菌种采集................................................................................................................................52.1.2实验试剂................................................................................................................................52.1.3实验仪器................................................................................................................................52.1.4培养基....................................................................................................................................62.2研究方法.......................................................................................................................................72.2.1菌种富集培养........................................................................................................................72.2.2分离纯化..........................................................
