鸡的大小黄白卵泡的分离和总RNA提取分析研究 生物技术专业

鸡的大小黄白卵泡分离及总 RNA 的提取目录中文摘要：................................................................................................2关键词：....................................................................................................2Abstract：.................................................................................................2前言............................................................................................................21.材料和方法............................................................................................41.1 实验动物及其组织.......................................................................41.2 主要仪器和试剂...........................................................................41.2.1 实验仪器.............................................................................41.2.2 实验试剂.............................................................................41.2.3 溶液配制.............................................................................51.3 实验操作步骤...............................................................................51.3.1 取样.....................................................................................51.3.2 大小黄白卵泡的提取.........................................................61.3.3 总 RNA 的提取.....................................................................61.3.4 总 RNA 完整性鉴定..............................................................61.3.5 总 RNA 内基因组 DNA 的去除.............................................61.3.6 反转录反应.........................................................................71.3.7 PCR 反应.............................................................................71.3.8 电泳检测............................................................................71.3.9 PCR 产物纯化.....................................................................71.3.10 连接转化..........................