学号：05439112江苏工业学院毕业设计（论文）（2009届）题目脂肪醇聚氧乙醚降解菌的筛选和鉴定学生成效江学院（系）化学化工学院专业班级生物工程051校内指导教师王利群专业技术职务副教授校外指导老师专业技术职务二○○九年六月摘要脂肪醇聚氧乙烯醚降解菌的筛选和鉴定摘要：随着现代生物技术的发展，微生物在环境保护方面的应用前景越来越广阔。本研究通过用筛选脂肪醇聚氧乙烯醚（alkylalcoholpolyoxyethyleneether,AEO）降解菌的方法，对我国工业中用量最大的非离子表面活性剂AEO进行降解特性研究。从常州市清潭污水处理厂采集活性污泥，通过富集和划线培养获得两株对AEO有较强降解能力的纯培养A-1和A-2。根据其形态特征、培养特征和生理生化特征等，经初步鉴定为假单胞菌属。当温度为30℃、pH7.0、接种量为5%、AEO浓度为0.5‰时，培养108小时后，用KI-I2分光光度法测定其降解能力，两株菌株的降解效率均达到90%。关键词：脂肪醇聚氧乙烯醚；筛选；鉴定；生物降解ScreeningandidentificationofbacterialstrainsfordegradingAlkylalcoholpolyoxyethyleneetherAbstract:Withthedevelopmentofmodernbiotechnology,andmoremicroorganismhaveamoreandmorebroadrangeofapplicationsinenvironmentdisposal.Inthispaper,characteristicsofbacteriadegradingalkylalcoholpolyoxyethyleneether(AEO),whichisthemostpopularusednonionicsurfactantinourindustry,werestudiedbyscreeningscreeningmicroorganismswhichcoulddegradeAEOthroughslectivemediumforAEO.SludgesamplewerecollectedfromwastewatertreatmentplantinQingtanofChangzhou.TwostrainsnamedA-1andA-2whichhavethestrongcompetenceofdegradingwereselectedfromabovesludgebyenrichmentandpurification,culturecharacteristics,andphysiologicalandbiochemicalreactions,A-1andA-2wasidentifiedasPseudomonassp.ThedegradationabilitytoAEOoftwostrainswhichIhadscreenedwasdeterminedbyspectrophotometerwithKI-I2solutionattheconcentrationof1%.ThedegrationratesforAEObothreachto90%andconcentrationforAEOat500mg/Lwithin108hoursat30℃,pH7.0,withinoculatingquantityat5%.Keywords:alkylalcoholpolyoxyethyleneether(AEO);screening;identification;biodegradation目录摘要..........................................................................................................................................I目录.......................................................................................................................................III1前言........................................................................................................................................11.1研究背景.....................................................................................................................11.2国内外研究现状.........................................................................................................21.2.1泡沫分离方法..................................................................................................21.2.2膜分离法...........................................................................................................21.2.3凝处理法...........................................................................................................21.2.4吸附处理法.......................................................................................................21.2.5催化氧化处理法...............................................................................................21.2.6生物方法的现状与问题...................................................................................31.2.7发展趋势—复极性固定床电解法...........................
