ISSN1007-7626CN11-3870/Q中国生物化学与分子生物学报ChineseJournalofBiochemistryandMolecularBiology2010年3月26(3):215~223·综述·收稿日期:2009-10-08;接受日期:2009-11-09教育部科学技术研究重点项目(No.208111),海南省自然科学基金(No.80610)和海南省教育厅高等学校科研项目(No.Hj200702)*联系人Tel:86-0898-66180255;E原mail:jiping.zheng@gmail.comReceived:October8,2009;Accepted:November9,2009SupportedbyKeyProjectofChineseMinistryofEducation(No.208111),NaturalScienceFoundationofHainanProvince(No.80610)andHigherEducationProgramofHainanEducationalCommittee(No.Hj200702)*CorrespondingauthorTel:86原0898原66180255;E-mail:jiping.zheng@gmail.comVicRK(YycFG)双组分调节系统郑继平1),2)*,郭桂英2),3),林丛1)(1)海南大学农学院,海口570228;2)海南大学热带生物资源教育部重点实验室,海口570228;3)海南大学教务处,海口570228)摘要细菌双组分调节系统,或称之为双组分信号转导系统,是细菌感应外界多变环境,维持自身存活和生长繁衍的重要感应系统.在这些调节系统中,最早发现于枯草芽孢杆菌的VicRK(YycFG)系统因与细胞存活密切相关而倍受关注.该系统存在于少数低G+C含量的革兰氏阳性菌中,包括金黄色葡萄球菌和肺炎链球菌等致病菌,高度保守.许多证据显示,VicRK(YycFG)具有调控细胞壁合成与代谢、胞膜完整、细胞分裂、脂类代谢、多糖合成与被膜形成以及细菌毒力等多种功能,参与细胞的生长、分裂与感染.该系统异常可导致细菌生活力严重下降,甚至死亡,因而成为防治该类病原菌的重要靶标.关键词双组分调节系统;VicRK(YycFG);病原菌防治中图分类号Q933VicRK(YycFG)Two-ComponentRegulatorySystemZHENGJi-Ping1),2)*,GUOGui-Ying2),3),LINCong1)(1)CollegeofAgriculture,HainanUniversity,Haikou570228,China;2)KeyLaboratoryofTropicalBiologicalResources,MinistryofEducation,HainanUniversity,Haikou570228,China;3)AcademicAffairsOffice,HainanUniversity,Haikou570228,China)AbstractBacterialtwo-componentsystems,alsoreferredtoastwocomponentsignaltransductionsystems,arerecognizedasaubiquitousmechanismensuringthesurvivalandprosperityofabacteriumunderawidevarietyofenvironmentalconditions.Oftheindividualtwo-componentregulatorysystems,theVicRK(YycFG),originallyidentifiedinBacillussubtilis,hasexcitedinterestasessentialtwo-componentsystemsforcellgrowth,celldivisionandinfection.Theessentialityhasbeenreportedtoitscontrolofcellwallbiosynthesisandmetabolism,cellmembraneintegrity,celldivision,lipidintegrity,exopolysaccharidebiosynthesisandbiofilmformation,andvirulencefactorexpression.Recentlymanystudieshaveshownthatevensmallchangeofwhichmaycauseasignificantdeclineincellvirulenceandviability,eventodeath.BecauseoftheseeffectsonessentialfunctionsandthefactthattheYycFGtwo-componentregulatorysystemishighlyconservedinlowG+Cgram-positivebacteria,includingseveralmajorpathogens,forexampleStaphylococcusaureusandStreptococcusPneumoniae,VicRK(YycFG)hasbeenconsideredahopefulpotentialtargetforanti-infectivetherapeutics.Keywordstwo-componentsystem;VicRK(YycFG);pathogentherapy中国生物化学与分子生物学报第26卷作为感应外界环境变化和调控内部基因表达的“分子开关”,双组分调节系统(two-componentregulatorysystem,TCS)是细胞应对瞬息万变的外部环境,维持自身存活和调整毒力的重要信号传递系统,普遍存在于原核和真核细胞中.该系统包括两个基本组分:传感器蛋白(sensor)和应答调节蛋白(responseregulatorprotein,RRorRRP).传感器蛋白为膜相关组氨酸蛋白激酶(histidineproteinkinase,HKorHPK),RR为胞内反式作用因子.其作用机制是在外界环境信息的刺激下,胞膜上的HK自身组氨酸磷酸化活化,形成同源二聚体,与胞内游离的RR...