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Sub-Cell®GTAgaroseGelElectrophoresisSystemsInstructionManualCatalog#170-4401to170-4406170-4481to170-4486WarrantyBio-RadLaboratorieswarrantstheSub-CellGT,WideMini-Sub®CellGT,andMini-SubCellGTelectrophoresissystemsagainstdefectsinmaterialsandworkmanshipfor1year.Ifanydefectsoccurintheinstrumentduringthiswarrantyperiod,Bio-RadLaboratorieswillrepairorreplacethedefectivepartsfree.Thefollowingdefects,however,arespecificallyexcluded:1.Defectscausedbyimproperoperation.2.RepairormodificationdonebyanyoneotherthanBio-RadLaboratoriesoranauthorizedagent.3.UseoffittingsorothersparepartssuppliedbyanyoneotherthanBio-RadLaboratories.4.Damagecausedbyaccidentormisuse.5.Damagecausedbydisaster.6.Corrosionduetouseofimpropersolventorsample.Thiswarrantydoesnotapplytopartslistedbelow:1.PlatinumElectrodeWiresToinsurethebestperformancefromtheSub-CellGTelectrophoresissystems,becomefullyacquaintedwiththeseoperatinginstructionsbeforeuse.Bio-Radrecommendsthatyoufirstreadtheseinstructionscarefully.Assembleanddisassembletheunitcompletelywithoutcastingagel.Afterthesepreliminarysteps,youshouldbereadytocastandrunagel.Bio-RadalsorecommendsthatallSub-CellGTsystemcomponentsandaccessoriesbeinspectedfordamage,cleanedasrecommendedinthismanual,andrinsedthoroughlywithdistilledwaterbeforeuse.Recordthefollowingforyourrecords:Foranyinquiryorrequestforrepairservice,contactBio-RadLaboratoriesafterconfirmingthemodelandserialnumberofyourinstrument.Model__________________________________________________CatalogNo._____________________________________________DateofDelivery_________________________________________WarrantyPeriod________________________________________SerialNo._______________________________________________InvoiceNo.______________________________________________PurchaseOrderNo._____________________________________TableofContentsPageSection1GeneralInformation11.1Introduction11.2Safety11.3SystemComponents21.4Specifications4Section2OperatingInstructions42.1DNAGelPreparation42.2CastingAgaroseGelSlabs62.3Electrophoresis82.4NucleicAcidStainingandVisualization92.5NoteonBlotting10Section3GelandElectrophoresisReagentPreparation10Section4CareandMaintenance114.1CleaningSub-CellGTComponents114.2CompatibleCleaningAgents114.3MaintenanceSchedule124.4ElectrodeReplacement124.5RNaseDecontamination13Section5Troubleshooting13Section6ProductInformation146.1Sub-CellGTSystems146.2Sub-CellGTSystemAccessories156.3RelatedBio-RadProducts17Section7References19Section1GeneralInformation1.1IntroductionTheSub-Cell®GTinstruments(basicSub-Cell®GTcell,WideMini-Sub®CellGT,andMini-SubCell®GT)compriseacomprehensiveandflexiblegelelectrophoresissystemthateffectivelyseparatesnucleicacidsusingsubmergedagarosegels.Submarineagarosegelsareeasytocastandreadilydissipateheat.Thesegelsallowsampleunderlayingandpreventelectricalfielddiscontinuitiescausedbywicksorsamplewelldehydration.AgarosegelsareidealfortheseparationofDNArestrictiondigestions,polymerasechainreaction(PCR*)-amplifiedfragments,andgenomicDNAandRNApriortoSouthernornorthernblotting.Ifoperatedcorrectly,agarosegelsubmarineelectrophoresiscaneffectivelyseparatenucleicacidsfrom20basepairsto20kilobasepairsinlength.TheSub-CellGTsystemsaredesignedforyearsofreproducibleandrigoroususe.Theseruggedsystemsincorporatemanyfeaturesthatmakecastingandrunningagarosegelssimpleandefficient.Thegelcasterprovidest...