河北工大学毕业论文作者：贾晋阳学号：学院：化工学院系(专业)：制药工程题目：甘草酸的纯化工艺研究指导者：(姓名)(专业技术职务)评阅者：(姓名)(专业技术职务)2012年6月9日毕业设计中文摘要甘草酸的纯化工艺研究摘要：从6种树脂中通过静态吸附筛选出了ADS-17树脂作为提取纯化甘草酸的最佳树脂。研究了pH值、上样液流速、上样液浓度、洗脱液浓度、洗脱液用量这五个因素对甘草酸吸附、解吸作用的影响，并通过正交试验考察了最佳工艺条件。实验结果证明最佳吸附条件为：pH值为6.0、上样液流速为2BV/h、上样浓度为10mg/ml；最佳解吸条件为：洗脱剂10%乙醇、洗脱液用量234ml。在实验得出的最佳条件下，甘草酸的纯度为65.07%，回收率为61.39%。另外，我们还在氢氧化钠回流的条件下进行了甘草酸构型的转换，结果表明转构后的甘草酸纯度为87.66%，产率44.1%。关键词：大孔树脂吸附纯化甘草酸毕业设计外文摘要TitleTheresearchofthePurificationTechnologyofGlycyrrhizicAcidAbstractBystaticadsorption,ADS-17resinisfilteredastheoptimalresintoextractpurifiedglycyrrhizicacidfromthesixkindsofresins.ThestudyofpH,supernatantflowrate,supernatantconcentration,eluentconcentrationandeluentamountshowsthesefivefactorseffectsontheadsorptionanddesorptionofglycyrrhizicacid,andoptimumconditionswereinvestigatedbyorthogonalexperiment.Experimentalresultsshowedthattheoptimumadsorptionconditionsasfollows:pH6.0,supernatantflowratefor2BV/h,theconcentrationofthesupernatantfor10mg/ml;bestdesorptionconditionswereasfollows:10%ethanol,eluentamountfor234ml.Underoptimalconditionsdrovedbyexperiment,thepurityofglycyrrhizicacidwas65.07%,recoveryratewas65.3%.Inaddition,wecompletedthestructuralconversionofglycyrrhizicacidunderaconditionofsodiumhydroxide'sreflux;resultsshowedthatthepurityofglycyrrhizicacidreached87.66%;recoveryratereached44.1%aftertheconversion.Keywords：MacroporousresinsAdsorptionPurificationGlycyrrhizicAcid目次1引言..................................................................11.1甘草酸简介............................................................11.2甘草酸及其生产概述....................................................21.3大孔树脂分离纯化技术..................................................31.4本论文研究内容........................................................32实验原理及材料........................................................42.1实验原理..............................................................42.2实验试剂和设备........................................................53甘草酸的纯化研究......................................................73.1大孔树脂的预处理及再生................................................73.2甘草酸检测方法的确定..................................................83.3树脂种类的选择........................................................93.4甘草酸的动态吸附.....................................................113.5甘草酸的梯度洗脱.....................................................143.618β-甘草酸到18α-甘草酸的构型转换.....................................184实验数据与讨论.......................................................194.1静态实验结果分析.....................................................204.2动态试验结果分析.....................................................204.3构型转换结果分析.....................................................20结论....................................................................22参考文献...............................................................23致谢.......................................
