荧光光谱法在中药品级鉴别中的应用研究摘要本课题运用FLS920型稳态和瞬态荧光光谱仪来测量在不同蒸煮时间下的当归和独活的荧光光谱，通过观察它们的荧光光谱来鉴别和区分出当归和独活这两种中药。通过实验结果可以发现，当归对波长为348nm的光较为敏感，而独活对波长为349nm的光较为敏感；两者的最高峰的高度都随着蒸煮时间的增加而降低，当归的峰高变化的幅度大，而独活的峰高变化幅度小；当归的荧光光谱的宽度较小，而独活的荧光光谱的宽度较大；独活的最高峰的位置在当归的最高峰的右边;当归的荧光光谱曲线较陡，而独活的荧光光谱曲线较为平缓。本研究结果对于鉴别当归和独活有一定的参考价值。该论文有图23幅，表2个，参考文献12篇。关键字：中药当归独活荧光光谱RadixAngelicaeSinennsisandHeracleumhemsleyanumDielsfluorescencespectrometryidentificationAbstractBytestingthefluorescencespectrumofRadixAngelicaeSinennsisandHeracleumhemsleyanumDiels,improvingthetestingcandistinguishbetweenthetwokindsoftraditionalChinesemedicine(TCM)whetherornot.BytestingtheRadixAngelicaeSinennsis’sandHeracleumhemsleyanum’sexcitationspectrum,emissionspectrumandpolarizationspectrumunderdifferentcookingtime,theoperationwilltellusthatspectrumcharacteristicsofthetwokindsofChinesemedicinalmaterialsinthespectrumcurvechangeruleunderdifferentcookingtime.Underdifferentcookingtime,wewillfindthedifferenceinthethreeflourescencespectrumarehuge.Accordingtotheresultofdataanalysis,theRadixAngelicaeSinennsisisobviouslydifferentfromHeracleumhemsleyanumDielsinfluorescencespectrum.AndwecandistinguishtwoChinesemedicinesthroughthismethod.Keywords:ChinesemedicineRadixAngelicaeSinennsisHeracleumhemsleyanumDielsfluorescencespectrum目录摘要...............................................................................................................................................IAbstract.......................................................................................................................................II图清单..........................................................................................................................................III表清单..........................................................................................................................................IV变量注释表..................................................................................................................................V1.绪论..........................................................................................................................................11.1鉴别中药方法.................................................11.2研究对象介绍.................................................22实验的基本原理.......................................................................................................................42.1分子荧光的产生...............................................42.2影响分子荧光的因素..........................................42.3荧光激发光谱和荧光发射光谱...................................53实验..............................................................................................................................................74.实验结果和分析...................................................................................................................104.1当归的光谱分析..............................................104.2独活的荧光光谱分析.........................................144.3当归与独活的荧光光谱的...
