课题名称生化标志物检测在急性心肌梗死（AMI）诊断中的应用目录中文摘要.....................................................................................................................Ⅰ英文摘要....................................................................................................................Ⅱ前言..............................................................................................................................11对象与方法.............................................................................................................21.1对象.....................................................................................................................21.2检验方法...........................................................................................................21.3统计分析...........................................................................................................32结果.........................................................................................................................42.1一般结果...........................................................................................................42.2AMI各期CK-MB、T(cTnT)水平的比较.....................................................42.3CK-MB与T(cTnT)的Logistic回归分析结果...........................................63讨论.........................................................................................................................84结论.......................................................................................................................10参考文献...................................................................................................................11致谢............................................................................................................................13综述............................................................................................................................14摘要目的:讨心肌生化标志物肌酸激酶同工酶(CK-MB)、肌钙蛋白T(cTnT)在急性心肌梗死(AMI)早期诊断中的临床应用，为急性心肌梗死患者的诊治提供可靠的理论依据。方法:收集湖南中医药研究院附属医院检验科AMI患者和健康体检者共计200例患者、100例AMI患者以及100例健康体检者的血清，采用免疫抑制法检测血清中的肌酸激酶MB同工酶(CK-MB)和肌钙蛋白T(cTnT)。结果:1、AMI患者组血清CK-MB、T(cTnT)水平显著高于对照组（P＜0.01），且AMI组升高幅度更为明显；2、AMI组中，CK-MB、T(cTnT)水平随病情发展而显著升高（P＜0.01）；3、CK-MB、T(cTnT)在AMI组的曲线下面积（areaundercurve,AUC）分别为0.56、0.75。结论:本研究结果显示，CK-MB、T(cTnT)对AMI早期有一定的诊断价值，可预示AMI疾病的发生、估计梗死范围、及早对患者做出治疗,有效地降低病死率。关键词：急性心肌梗死；心肌生化标志物；肌酸激酶同工酶；肌钙蛋白TAbstracObjective:Toinvestigatetheclinicalapplicationofcreatinekinaseisoenzyme(CK-MB)andtroponinT(cTnT)intheearlydiagnosisofacutemyocardialinfarction(AMI),andtoprovidereliabletheoreticalbasisforthediagnosisandtreatmentofacutemyocardialinfarction.Methods:AMIpatientsandhealthysubjectswereenrolledinatotalof200patients,40patientswithAMIand50healthysubjects.TheserumcreatinekinaseMBisoenzymewasdetectedbyimmunosuppressivemethod.CK-MB)andtroponinT(cTnT).Results:ThelevelofCK-MBandT(cTnT)intheAMIgroupwassignificantlyhigherthanthatinthecontrolgroup(P<0.01),andtheelevationoftheAMIgroupwasmoreobvious...
