题目:石斑鱼神经坏死病毒的抗原制备目录【摘要】........................................................................................................................1【关键词】...............................................................Error:Referencesourcenotfound1.前言............................................................................................................................11.1水产养殖的现状及问题...................................................................................11.2神经坏死病毒...................................................................................................21.3神经坏死病毒衣壳蛋白...................................................................................22.实验材料与试剂.........................................................................................................32.1实验材料...........................................................................................................32.1.1实验菌株、载体与ULP酶....................................................................32.1.2主要仪器与设备.....................................................................................32.2实验试剂及主要试剂的配制...........................................................................32.2.1实验试剂.................................................................................................32.2.2主要试剂的配制.....................................................................................43.实验方法及过程........................................................................................................43.1连接PET-SUMO载体......................................................................................53.2连接产物转化BL21菌株测序鉴定重组子....................................................53.3IPTG诱导GNN菌株诱导................................................................................63.4诱导表达蛋白提取...........................................................................................63.5总蛋白SDS-PAGE胶检测表达结果..............................................................63.5.1制胶.........................................................................................................73.5.2加样与电泳.............................................................................................73.6亲和层析纯化目的蛋白...................................................................................73.7ULP酶切............................................................................................................83.8纯化酶切蛋白...................................................................................................83.9质谱鉴定目的片段...........................................................................................84.实验结果与分析........................................................................................................94.1石斑鱼神经坏死病毒衣壳蛋白的基因重组子测序.......................................94.2融合蛋白SUMO-gNNVcp的诱导表达........................................................104.3融合蛋白酶切纯化.........................................................................................114.4目的蛋白gNNVcp的质谱鉴定.....................................................................115.讨论..................................
