目录中文摘要..................................................................................................................1英文摘要..................................................................................................................4前言..........................................................................................................................7第一章芍药苷治疗肠易激综合征的药效学研究.................................................91.材料....................................................................................................................91.1仪器..................................................................................................................91.2药品与试剂......................................................................................................91.3实验动物..........................................................................................................92.实验方法..........................................................................................................102.1大鼠腹泻模型的建立与药物治疗................................................................102.2小鼠腹痛模型的建立与药物治疗................................................................103.结果..................................................................................................................123.1芍药苷改善大鼠腹泻症状............................................................................123.2芍药苷治疗小鼠腹痛症状............................................................................124.讨论..................................................................................................................13第二章芍药苷对肠上皮细胞膜屏障的保护作用及机制探讨...........................151.材料..................................................................................................................151.1仪器................................................................................................................151.2药品和试剂....................................................................................................161.3实验动物和细胞株........................................................................................182.实验方法..........................................................................................................182.1Caco-2细胞正常屏障模型的构建...............................................................182.2Caco-2细胞屏障功能紊乱模型的构建.......................................................202.3芍药苷对Caco-2细胞屏障功能的保护作用..............................................212.4芍药苷对Caco-2细胞紧密连接蛋白ZO-1表达的影响............................222.5芍药苷对Caco-2细胞存活率的影响..........................................................263.结果..................................................................................................................273.1Caco-2细胞正常和功能紊乱屏障模型.......................................................273.2芍药苷对Caco-2细胞屏障功能的保护作用..............................................303.3芍药苷对Caco-2细胞紧密连接蛋白ZO-1表达的影响............................313.4芍药苷对Caco-2细胞存活率的影响..........................................................324.讨论.......................................
