pGEX-4T-1 编号 载体名称 北京华越洋生物VECT4940 pGEX-‐4T-‐1 pGEX4T1载体基本信息 别名: pGEX-‐4T-‐1, pGEX4T1, pGEX 4T 1 质粒类型: 大肠杆菌蛋白表达载体 表达水平: 高拷贝 启动子: Tac 克隆方法: 多克隆位点，限制性内切酶 载体大小: 4969 bp 5' 测序引物及序列: pGEX5': GGGCTGGCAAGCCACGTTTGGTG 3' 测序引物及序列: pGEX3': CCGGGAGCTGCATGTGTCAGAGG 载体标签: N-‐GST 载体抗性: Ampicillin 氨苄 备注: 复制子是pMB1，限制性内切酶酶切位点有 BamHI,EcoRI SmaI,Sal I,Xho I,Not I 等，可转化进大肠杆菌中高拷贝表达。pGEX-‐4T-‐1载体是一种常用的原核表达载体，该载体多克隆位点区域含有多个常用的内切酶位点序列，便于不同基因的克隆；且全序列中含有tac启动子、GST 标签序列，是一种高效的蛋白表达载体。Tac启动子在没有诱导物存在的情况下，质粒上携带的lacIq基因产物可以有效抑制tac启动子的转录。可在BL21、Rosstea等表达菌株中高表达融合有GST 标签的蛋白，且用凝血酶可将目的蛋白的GST 标签切掉，便于下游蛋白纯化。 稳定性: 瞬时表达 Transient 组成型: 诱导表达 病毒/非病毒: 非病毒 pGEX4T1载体质粒图谱和多克隆位点信息 pGEX4T1载体简介 There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-‐well plates. For sample volumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available comb...
