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-------------学院本科生毕业论文题目连翘及桑葚中芦丁含量的测定专业应用化学院部化学与化学工程学院学号、、、姓名、、、指导教师、、教授答辩时间、、、论文工作时间：2011年12月至2012年5月论文题目来源：国家自然科学基金项目编号：四川省自然科学研究项目编号：校级自然科学研究项目编号：连翘及桑葚中芦丁含量的测定学生--------指导教师--------摘要：利用反相高效液相色谱法测定连翘及桑葚中芦丁成分的含量。采用SHIMDZUVP-ODS(4.6mm×150mm、5.0μm)色谱柱，甲醇-0.08%：硫酸溶液(6:4)为流动相，流量为1.0mL·mol-1，检测波长为360nm。结果：芦丁标准溶液在0.464-2.32µg范围内的线性回归方程为Y=21200X+13697(r=0.9995)，平均回收率为99.4%，RSD=0.8%(n=5)。结论：此方法可用于检测连翘及桑葚中芦丁的含量，为连翘及桑葚的质量评价提供依据。实验表明该方法简便、快捷、适用性好。关键词：HPLC；连翘；桑葚；芦丁；含量测定Determinationofrutininforsythiasuspense(thunb.)andmulberryUndergraduate-----------Supervisor-------------------Abstract：ThepaperistointroducetheexperimentofdeterminingthecontentsofrutininForsythiasuspense(Thunb.)andmulberrywithRP-HPLC.TakingSHIMDZUVP-ODSconlumn(4.6mm×150mm,5.0μm),amixtureofmethanol-0.08%phosphoricacidintheratioof6to4byvolumewithaflow-rateof1.0mL·mol-1andthedetectionwavelengthwassetupat360nm.Results:thelinearityofrutinwasobtainedovertheranges0.464-2.32µg.ThelinearequationwasY=21200X+13697(r=0.9995),theaveragerecoveryratewas99.4%,RSD=0.8%(n=5).ThemethodcanbeusedforthedeterminationofrutininForsythiasuspenseandmulberry,whichprovidesascientificbasisforqualityassessmenttoForsythiasuspenseandmulberry.Thismethodisreliableaccurateandsuitableforthedeterminationofrutin.Keywords：HPLC;Forsythiasuspense;Mulberry;Rutin;Determination目录前言....................................................................11连翘、桑葚、芦丁及高效液相色谱法的介绍........................................................................11.1连翘的简介......................................................................................................................11.2桑葚的简介......................................................................................................................11.3芦丁的简介......................................................................................................................11.4高效液相色谱的介绍......................................................................................................22实验部分....................................................................................................................................32.1仪器与试剂......................................................................................................................32.2仪器工作条件..................................................................................................................42.3样品制备..........................................................................................................................42.3.1标准储备液的制备...............................................................................................42.3.2供品溶液的制备...................................................................................................42.4高效液相色谱法测量......................................................................................................52.4.1流动相的准备.................................................................................