分子生物学试验常用缓冲液的配制方法

各种缓冲液的配制方法Na2HPO4-柠檬酸钠缓冲液PH2.22.42.62.83.03.23.43.63.84.04.24.44.64.85.00.2mol/LNa2HPO40.1mol/L 柠 檬 酸(ml)钠 (ml)0.419.61.242.183.174.114.945.706.447.107.718.288.829.359.8610.3018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.70PH5.25.45.65.86.06.26.46.66.87.07.27.47.67.88.00.2mol/LNa2HPO4(ml)10.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.450.1mol/L 柠檬酸钠(ml)9.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPO4。2H2O，分子量=178.050.2mol/L 溶液含 35.61g/L柠檬酸. H2O，分子量=210.140.1mol/L 溶液含 21.01g/LPH0.1mol/L 柠檬酸（ml）3.018.63.23.43.63.84.04.24.44.64.817.216.014.914.013.112.311.410.39.2柠檬酸-柠檬酸钠缓冲溶液（0.1mol/L）PH0.1mol/L 柠檬酸0.1mol/L 柠檬酸钠（ml）1.42.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.6（ml）8.27.36.45.54.73.82.82.01.40.1mol/L 柠檬酸钠(ml)11.812.713.614.515.316.217.218.018.6柠檬酸. H2O，分子量=210.140.1mol/L 溶液含 21.01g/L柠檬酸钠. 2H2O，分子量=294.12；0.1mol/L 溶液含 29.4 g/L醋酸缓冲液（0.2mol/L，18℃）PH3.63.84.04.24.44.60.2mol/LNaAC(ml)0.751.201.802.653.704.900.2mol/LHAC(ml)9.258.808.207.356.305.10PH4.85.05.25.45.65.80.2mol/LNaAC(ml)5.907.007.908.609.109.400.2mol/LHAC(ml)4.103.002.101.400.900.60NaAC.3 H2O,分子量=136.090.2mol/L 溶液含 27.22g/L（1）醋酸盐溶液的配制：醋酸－醋酸钠缓冲液(pH3.6)取醋酸钠 5.1g,加冰醋酸 20ml，再加水稀释至 250ml,即得。醋酸－醋酸钠缓冲液(pH3.7)取无水醋酸钠 20g,加水 300ml 溶解后，加溴酚蓝指示液 1ml 及冰醋酸 60～80ml，至溶液从蓝色转变为纯绿色，再加水稀释至 1000ml，即得。醋酸－醋酸钠缓冲液(pH3.8)取 2mol/L 醋酸钠溶液 13ml 与 2mol/L 醋酸溶液 87ml,加每 1ml 含铜 1mg 的硫酸铜溶液0.5ml,再加水稀释至 1000ml，即得。醋酸－醋酸钠缓冲液(pH4.5)取醋酸钠 18g,加冰醋酸 9.8ml,再加水稀释至 1000ml,即得。醋酸－醋酸钠缓冲液(pH4.6)取醋酸钠 5.4g,加水 50ml 使溶解，用冰醋酸调节 pH 值至 4.6，再加水稀释至 100ml，即得。醋酸－醋酸钠缓冲液(pH6.0)取醋酸钠 54.6g，加 1mol/L 醋酸溶液 20ml 溶解后，...