生 物 分子类实验室常用实验技术原理汇总 一、GST pull-down 实验 ................................................................................................. 2 二、足印法(Footprinting) ........................................................................................... 2 三、染色质免疫共沉淀技术(Chromatin Immunoprecipitation,ChIP) ................... 2 四、基因芯片(又称 DNA 芯片、生物芯片)技术 ................................................... 2 五、高效液相色谱(HPLC) ......................................................................................... 3 六、酵母双杂交(Y2H) ............................................................................................... 5 七、噬菌体展示技术 ....................................................................................................... 8 八、RNA 提取(Trizol 法) ........................................................................................... 9 九、RT-PCR ................................................................................................................... 11 十、实时荧光定量 PCR(Q—PCR)(Real-time Quantitative PCR ) ..................... 11 十一、BCA 法测蛋白质浓度 ........................................................................................ 13 十二、大肠杆菌感受态细胞(E.coli DH5α )制备 ................................................... 13 十三、碱变性提取质粒 DNA ....................................................................................... 14 十四、目的基因的连接、转化及克隆筛选 ................................................................. 14 十五、RNA 干扰(RNAi) .......................................................................................... 16 十六、cDNA 末端快速扩增 (rapid amplification of cDNA ends,RACE)技术 .......... 17 十七、基因文库和 cDNA 文库的构建(看课本上的) ............................................. 19 十八、mRNA 差异显...