精品文档---下载后可任意编辑mir-663 通过靶向 TGF-beta1 调控细胞辐射应答的开题报告摘要：本文旨在讨论 microRNA-663（mir-663）在肺癌细胞辐射应答中的调控作用及其机制。通过生物信息学分析和文献讨论，发现 mir-663可能通过靶向 TGF-beta1 在肺癌细胞辐射应答中发挥重要作用。进一步实验发现，辐射处理后 mir-663 表达水平显著升高，同时 TGF-beta1 mRNA 和蛋白表达水平也有所上调，说明 mir-663 可能通过抑制 TGF-beta1 表达来调节肺癌细胞辐射应答。最后，通过细胞增殖和凋亡实验发现，mir-663 的上调能够促进辐射治疗后肺癌细胞的凋亡和抑制细胞增殖。关键词：mir-663、TGF-beta1、肺癌、辐射、细胞凋亡Abstract:This study aims to investigate the regulatory role and mechanism of microRNA-663 (mir-663) in the radiation response of lung cancer cells. Through bioinformatics analysis and literature research, it was found that mir-663 may play an important role in the radiation response of lung cancer cells by targeting TGF-beta1. Further experiments showed that the expression level of mir-663 was significantly increased after radiation treatment, and the mRNA and protein expression levels of TGF-beta1 were also up-regulated, indicating that mir-663 may regulate the radiation response of lung cancer cells by suppressing the expression of TGF-beta1. Finally, it was found that the up-regulation of mir-663 can promote the apoptosis of lung cancer cells after radiation therapy and inhibit cell proliferation through cell proliferation and apoptosis experiments.Keywords: mir-663, TGF-beta1, lung cancer, radiation, apoptosis
