精品文档---下载后可任意编辑OsGATAs 参加 BR 信号调控水稻株型和种子发育的机制讨论中期报告摘要：OsGATAs 是一类编码调控细胞分化和组织发育的转录因子，其在植物生长发育过程中起重要的调控作用。在本讨论中，我们探究了OsGATAs 在植物生长发育过程中参加 BR 信号调控水稻株型和种子发育的机制。讨论结果显示，在 12 种 OsGATAs 基因中，OsGATA11 和 OsGATA26 的表达水平在水稻的发育过程中显著升高。利用基因克隆和转基因水稻的技术，我们进一步验证了 OsGATA11 和 OsGATA26 对 BR 信号在调控水稻株型和种子发育中的作用。实验结果表明，OsGATA11 和 OsGATA26 的过表达可以显著增加水稻的株高、叶片大小、花药长度和种子大小，并且提高种子成熟率。结合基因表达分析和转录因子启动子序列分析，我们确定了 OsGATA11 和 OsGATA26 的调控机制，发现其与 BR 信号的感受器 BIN2 之间存在互作关系，并且可以结合 BR 累加物 BES1 和 BZR1 来增强 BR 的信号响应。本讨论的结果为深化解析 OsGATAs 转录因子在植物生长发育中的调控机制提供了参考。关键词：OsGATAs；BR 信号；水稻株型；种子发育；BIN2；BES1；BZR1Abstract:OsGATAs are a family of transcription factors that regulate cell differentiation and tissue development in plants, and play an important regulatory role in plant growth and development. In this study, we investigated the mechanism of OsGATAs involved in BR signal regulation of rice plant type and seed development. The results showed that the expression levels of OsGATA11 and OsGATA26 were significantly increased during rice development among 12 OsGATAs genes. By using gene cloning and transgenic rice technology, we further verified the role of OsGATA11 and OsGATA26 in regulating BR signal in rice plant type and seed development. The experimental results showed that overexpression of OsGATA11 and OsGATA26 could significantly increase the plant height, leaf size, stamen length, and seed size of rice, and improve the seed maturity rate. Combined with gene expression analysis and transcription factor promoter sequence analysis, we 精品文档---下载后可任意编辑determined the regulatory mechanism of OsGATA11 and OsGATA26, found that they interact with the BR signal receptor BIN2, and can bind with BR accumulations BES1 and BZR1 to enhance the signal response to BR. The results of this study provide a reference for in-depth analysis of the regulatory mechanism of OsGATAs transcription factors in plant growth and development.Keywords: OsGATAs; BR signal; rice plant type; seed development; BIN2; BES1; BZR1
