蛋白质的分离纯化I.Whichfactorisusedtoseparateproteinsbycentrifugation?a. lengthb. massc. charged. conformatione. enzymerate离心法依据什么因素来分离蛋白质?a:长度b.质量c.收费d■构象e.酶率2.Whichtechniqueisusedtodetectproteinbandsthat哪种技术被用来检测转移到膜上的蛋白条带?havebeentransferredtoamembrane?a.绿色荧光蛋白a.GFPb—抗b.primaryantibodyonlyC.二抗c.secondaryantibodyonlyd.显色反应d.chromogenicreactione.聚丙烯酰胺e.polyacrylamide3.Twoglobularproteinsofthesamemolecularweight在 pH 为 7.8 的缓冲液中,在 SDS-PAGE 凝胶中使用两个arerunoutonaSDS-PAGEgelusingabufferofpH7.8.相同分子量的球状蛋白。蛋白质 A 富含谷氨酸和天冬氨ProteinAisenrichedinglutamicandasparticacid酸残基,pl 为 4.5,而蛋白质 B 富含赖氨酸残基,pl 为residuesandhasapIof4.5,whereasproteinBis10.3。如果蛋白质在相邻的孔中同时运行,贝顾测结果。abundantinlysineresiduesandhasapIof103Predicta.无法事先确定结果,除非有更多的信息关于他们的四兀theoutcomeiftheproteinsarerunatthesametimein结构和非共价蛋白质的相互作用。adjacentwells.b.蛋白质 A 会在凝胶中进一步延伸。a.Youcannotdeterminetheoutcomebeforehandc.只有蛋白质 A 会沿着凝胶流向正极。withoutfirsthavingmoreinformationontheirquaternaryd•蛋白质将在凝胶中移动相同的距离。structureandnoncovalentproteininte「actions.e.蛋b.ProteinAwillrunfurtherdownthegel.白质 B 会在凝胶中进步延伸。c.OnlyProteinAwillrundownthegeltowardthepositiveelectrode.d.Theproteinswillrunthesamedistaneedownthegel.e.ProteinBwillrunfurtherdownthegel.SDS1.InSDS-polyacrylamidegelelectrophoresis,proteinsare在 sds-聚丙烯酰胺凝胶电泳中,蛋白质的分解是基于它resolvedbasedontheir:a.totalnegativecharge.b. molecularweight.c.shapeandcharge.d. shape.e. charge们:a•总负电荷。b•分子量。c.形状和电荷。d 形状。e.电荷2.SDScoatsproteinswithcharges.a.cationicb.positivec.Noneoftheaboveiscorrect.d.neutrale.negativeSDS 给蛋白质包裹上一层带着的电荷。a.阳离子B.阳性C.以上都不正确。d 中性E..阴性3.Whichstatementisfalse?a. WhenaproteinsamplecontainingSDSisheated,theSDSmoleculesbindtheproteinsandcau...