AAV动物实验--教你如何选择血清型,注射方法

AAVDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDAAVDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDAAVDDDAAVDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDODDAAV1DDDDDDDDDDDDDDDAAV2DDDDDAAV5DDDDDDDDDAAV6DDDDDDDDDDDDDDDAAV8DDDDDDDDDDDDAAV9DDDDDDDDDDDDDDDDJDDDDDDDDDJ/8DDDDDDDDDPHP.eB/PHP.B/PHP.SDDDDDDDDRetroDDDDDDDRhIODDDDDDDDDDDDDDDDDDDAAVDDDDDAAVDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDIntraperitonealInjectionsMUIPDDDDDDIntravenousInjectionsUMIVDDDDDIntramuscularInjectionsUMIMDDDDDSubcutaneousInjectionsUMSQDDDDDIntradermalInjectionsUMIDODDGavageDOG/IGDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDAnalysisofAAVSerotypes1D9MediatedGeneExpressionandTropisminMiceAfterSystemicInjectionUUMCICICICI[HTML]AnalysisofAAVserotypes1-9mediatedgeneexpressionandtropisminmiceaftersystemicinjectionCZincarelli;SSoltysG Ren go,JERabinowitz-MolecularTherapy,2C0S-ElsevierThisstudyexaminestransgeneexpressionandbiodistributionofadena-associatedviirns(AAV)pseudotyped1-9aftertailvsin(TV)injectioninmalemice.UsingacytdmegaIovirus(CMV)-luciferasetransgene;thetime-courseofexpressionineachanimalwastrackedthroughouttheexperiment.Theanimalswereimagedat7,14,29,56.and100daysaftertheTVinjection.Thetotalnumberofphotonsemittedfromeachanimalwasrecorded,allowingexaminationofexpressionlevelandkineticsforeachpseudotypedvirus.The...書 09 被引用次数：8 弭相关文童所有 14 个版本DDDDDDDDAAVODD1-9DDAAV-CMV-LucDDDDDDDDD-DDDDDD9DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDAAV2,3,4,5ULow-expressiongroulpaDDDDDDD5,000-25,000photons/second/cm2UAAVDDDD--DDDDDDDDDDDDDD15,000-200,000photons/second/cm2 口DDDDDDDDDDDDDDDDDDDDDDDDDAAV9DDDDDDDDDDDDDDDDDDDDDDDAAVDDDDDDDDDDDDDDDDDDDDDDAAVDDDDDDDLuciferaseDDDDDDDAAV1,6,8UMedium-expressiongroupDDDDDDDDDDDAAV9DDDDAAV4DDDDDDDDDDDDDDDDDDDDDDDAAV6DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDAAV7,9DHigh-expressiongroup(c)DDDDDD10,000-1,000,000photons/second/cm2 口25,000-20,00015.000•AAV1■AAVEBAAV3GAAV4O^AY59AAV6 口 AAV7■AAVS0MV9Ejucifersseproteinexpressi...