电脑桌面
添加小米粒文库到电脑桌面
安装后可以在桌面快捷访问

大黄鱼鼠多抗浅析

大黄鱼鼠多抗浅析_第1页
1/16
大黄鱼鼠多抗浅析_第2页
2/16
大黄鱼鼠多抗浅析_第3页
3/16
摘 要 IgM 是大黄鱼最主要的免疫球蛋白,在鱼体免疫系统中发挥重要作用。本论文以实验室前期利用 Protein A 亲和纯化的大黄鱼血清 IgM 为抗原,按照标准免疫流程免疫 Balb/C 小鼠,获取抗血清,即多克隆抗体。利用酶联免疫吸附方法(ELISA)测定鼠源抗血清的效价达到 1:20000,效果较好;利用免疫印迹(Western blotting)对大黄鱼血清 IgM 鼠源多抗的结合特性进行了分析,结果显示多抗能特异性结合 IgM 重链和轻链,表明实验制备的多抗特异性良好,可应用于后续实验研究。大黄鱼血清 IgM 鼠多抗的制备有助于加深对鱼体免疫系统的理解,也将为大黄鱼免疫机理及相关免疫检测研究奠定基础。关键词:大黄鱼;IgM;鼠多抗;ELISA;Western blottingAbstractIgM is the main immunoglobulin in large yellow croaker, which plays a vital role in the immune system of fish. In this thesis, the serum IgM of Larimichthys crocea purified by protein A affinity, according to the standard immune process, was used as antigen to immunize Balb/c mice, and the antiserum, polyclonal antibody, was acquired. The valence of mouse antiserum determined by enzyme-linked immunosorbent assay (ELISA) achieved 1:20000. Western blotting was used to analyze the binding characteristics of murine polyclonal antibodies against serum IgM of L. crocea. The results showed that the polyclonal antibodies could specifically combine with heavy and light chains of IgM L. crocea, which indicated that it prepared in this experiment had good specificity and could be applied to subsequent experimental studies. The preparation of rat polyclonal antibody against the serum IgM of L. crocea helps to understanding of the immune system of the fish, and will also lay a foundation for the study of the immune mechanism and related immune detection of L. crocea.Key words: Larimichthys crocea; IgM; mice polyclonal antibody; ELISA; Western blotting目 录1 引 言..........................

1、当您付费下载文档后,您只拥有了使用权限,并不意味着购买了版权,文档只能用于自身使用,不得用于其他商业用途(如 [转卖]进行直接盈利或[编辑后售卖]进行间接盈利)。
2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。
3、如文档内容存在违规,或者侵犯商业秘密、侵犯著作权等,请点击“违规举报”。

碎片内容

大黄鱼鼠多抗浅析

确认删除?
VIP
微信客服
  • 扫码咨询
会员Q群
  • 会员专属群点击这里加入QQ群
客服邮箱
回到顶部