多糖含量测定几个不一样方法比较 系 别: 信 息 学 院 专 业: 生 物 工 程 学 号: 姓 名: 指 导 教 师: 指导老师职称: 讲 师 多糖含量测定几个不一样方法比较 摘要：本文综述了多糖含量测定几个惯用方法，主要有苯酚-硫酸法、3, 5-二硝基水杨酸法(简称 DNS 法)、蒽酮-硫酸法、色谱法、红外光谱定量分析多糖法等。并对这些方法优缺点进行了分析和比较。这些方法可为多糖含量测定提供一定参考，并为多糖含量测定更深化讨论提供一定理论基础。 关键词：多糖；含量；测定；方法 A review of different methods to the determination of polysaccharides Abstract: Paper reviewed some different methods to the determination of polysaccharides, in it phenol-vitriol method, 3, 5-two nitro salicylic (DNS) method, anthrone-vitriol method, chromatography, infrared spectrum quantitative analysis and etc had been dealed with. And the advantages and disadvantages of these methods are analyzed and compared. It provided some related information and based theories to the determination of polysaccharides content. Key words：polysaccharides; content; determination; methods 目 录 汉字摘要............................................................................. 英文摘要............................................................................. 1 序言...............................................................................1 2化学法测定多糖含量.................................................................1 2.1 苯酚-硫酸法 .....................................................................1 2.2 3, 5-二硝基水杨酸法(简称 DNS 法). .................................................2 2.3 蒽酮-硫酸法 .....................................................................2 3 色谱法测定多糖含量讨论 ..........................................................3 3.1 气相色谱法(GC) ...................................................................3 3.2 液相色谱法(HPLC) .....................................
